Effects of nitrogen supplementation of the culture medium on the growth, total lipid content and fatty acid profiles of three microalgae (Tetraselmis subcordiformis, Nannochloropsis oculata and Pavlova viridis)
Effects of nitrogen supplementation of the culture medium on the growth, total lipid content and fatty acid profiles of three microalgae (Tetraselmis subcordiformis, Nannochloropsis oculata and Pavlova viridis)
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DOI：
10.1007/s10811-012-9846-9
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发表时间：
2013-02-01
影响因子：
3.3
通讯作者：
Yan, Jiaqi
中科院分区：
文献类型：
--
作者：
Huang, Xuxiong;Huang, Zhengzheng;Yan, Jiaqi
为了提高微藻作为生物柴油生产源的性能，在添加不同氮量（0、0.22、0.44、0.88和1.76 mmol N center dot L-1）的培养基中培养3种微藻（亚心形四藻SHOU-S 05、微拟球藻SHOU-S14和绿色巴夫藻SHOU-S16）。培养10 d后，测定了3种微藻的生长、总脂含量、脂肪种类和脂肪酸组成。结果表明，T. subcordiformis、拟心脉脉孢N.不施氮处理中，黄姑鱼和绿姑鱼最低（分别为0.014、0.033和0.018），随着施氮量的增加，黄姑鱼和绿姑鱼的生长量显著增加。以0.22mmol N中心点L ~（-1）处理的微藻总脂含量最高，分别为29.77%、35.85%和32.10%. subcordiformis、拟心脉脉孢N. oculata和P. viridis。在0.22 ~ 1.76 mmol N center dot L-1范围内，三种微藻的总脂含量和中性脂所占比例随氮素补充量的增加而显著降低。三种微藻的脂肪酸组成存在显著差异。脂肪酸谱的明显特征。亚心形藻18：3 N3含量较高（17.68- 22.22%）。而EPA（C20：5 n3）和C16脂肪酸在N. oculata和P. viridis。N.在氮限制培养基中，眼斑紫菜和绿球藻积累了较多的16碳脂肪酸，而多不饱和脂肪酸积累较少。因此，有限的氮素处理是改善草绿色滨藜和红球藻品质的有效途径。作为生物柴油的来源。
To improve the properties of microalgae as sources for biodiesel production, three microalgae (Tetraselmis subcordiformis SHOU-S05, Nannochloropsis oculata SHOU-S14 and Pavlova viridis SHOU-S16) were cultured in media supplemented with different amounts of nitrogen (0, 0.22, 0.44, 0.88 and 1.76 mmol N center dot L-1). The growth, total lipid contents, lipid classes and fatty acid profiles of the three microalgae were assayed after 10 days of cultivation. The results indicated that the specific growth rates of T. subcordiformis, N. oculata and P. viridis were lowest (0.014, 0.033 and 0.018, respectively) in the treatments without nitrogen supplementation and increased significantly with increasing nitrogen supplementation. The microalgae treated with 0.22 mmol N center dot L-1 had the highest total lipid contents, which were 29.77, 35.85 and 32.10 % in T. subcordiformis, N. oculata and P. viridis, respectively. The total lipid contents as well as the proportions of neutral lipid in the three microalgae decreased significantly with increasing nitrogen supplementation between 0.22 and 1.76 mmol N center dot L-1. The fatty acid profiles of the three microalgae were significantly different. The obvious characteristic of the fatty acid profile of T. subcordiformis was a high amount (17.68-22.22 %) of 18:3n3. However, EPA (C20:5n3) and C16 fatty acids were significantly high in N. oculata and P. viridis, respectively. N. oculata and P. viridis accumulated more 16-carbon fatty acids and fewer polyunsaturated fatty acids in nitrogen-limited media. It is therefore suggested that a limited nitrogen treatment is effective for improving the properties of P. viridis and N. oculata as sources for biodiesel.