Gonadotropin depression of adenosine triphosphate levels and interaction with adenosine in rat granulosa cells.
Gonadotropin depression of adenosine triphosphate levels and interaction with adenosine in rat granulosa cells.
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促性腺激素抑制大鼠颗粒细胞中三磷酸腺苷水平及其与腺苷的相互作用。
作者：
H. Billig;S. Rosberg
用荧光素酶法测定PMSG处理的未成熟大鼠体外培养的排卵前颗粒细胞的ATP水平。FSH和LH均能降低ATP水平，FSH作用更明显。腺苷使ATP水平提高约3倍，但促性腺激素的抑制作用不能被腺苷所克服。颗粒细胞对腺苷的摄取遵循米氏动力学，Km为15.9 +/- 3.6 μ M，最大速率为1.6 +/- 0.1 pmol/min × 10（5）个细胞。腺苷摄取的半衰期约为40 min。FSH处理细胞后，腺苷的最大摄取量从48 +/- 5降至30 +/- 1 pmol/10（5）个细胞。颗粒细胞的cAMP和孕酮的基础分泌略有增加，但腺苷单独显着。腺苷显著增强FSH刺激的cAMP分泌，但不增强孕酮分泌。非代谢的腺苷类似物，2-氯腺苷，不影响ATP水平或颗粒细胞分泌cAMP。这项研究证实了以前的观察，腺苷可以增加ATP水平，并放大性腺细胞对促性腺激素的反应。一个新的发现是颗粒细胞中的ATP水平被促性腺激素显著抑制。据推测，这种ATP的抑制可能是颗粒细胞代谢控制的一个因素。
Cellular ATP levels were measured with the luceferin-luciferase enzyme method in incubated preovulatory granulosa cells in vitro from PMSG-treated immature rats. The ATP levels were depressed by both FSH and LH, FSH being the more effective. Adenosine enhanced the ATP levels about 3-fold, but the depressive effects of gonadotropins could not be overcome by the addition of adenosine. Uptake of adenosine in granulosa cells followed Michaelis-Menten kinetics, with a Km of 15.9 +/- 3.6 microM and a maximum velocity of 1.6 +/- 0.1 pmol/min X 10(5) cells. The half-time for uptake of adenosine was about 40 min. The maximal uptake of adenosine was lowered from 48 +/- 5 to 30 +/- 1 pmol/10(5) cells by FSH treatment of the cells. The basal secretions of cAMP and progesterone from the granulosa cells were slightly but significantly enhanced by adenosine alone. Adenosine markedly enhanced FSH-stimulated cAMP secretion, but not progesterone secretion. A nonmetabolizable adenosine analog, 2-chloro-adenosine, did not affect the ATP levels or the secretion of cAMP from granulosa cells. This study confirms previous observations that adenosine can increase ATP levels and amplify the response to gonadotropins in gonadal cells. A novel finding is that the levels of ATP in granulosa cells are markedly depressed by gonadotropins. It is speculated that this depression of ATP may be a factor in the metabolic control of granulosa cells.
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嘌呤调节性腺细胞中 LH 的作用。
DOI：
10.1016/0022-4731(83)90013-4
发表时间：
1983
期刊：
Journal of steroid biochemistry
影响因子：
--
作者：
Behrman,HR;Polan,ML;Ohkawa,R;Laufer,N;Luborsky,JL;Williams,AT;Gore,SD
通讯作者：
Gore,SD
腺嘌呤衍生的嘌呤会增加黄体细胞中三磷酸腺苷 (ATP) 的水平：证据表明细胞中的 ATP 水平可能会限制促黄体激素对腺苷 3,5-单磷酸积累的刺激。
影响因子：
4.8
作者：
Brennan,TJ;Ohkawa,R;Gore,SD;Behrman,HR
通讯作者：
Behrman,HR
嘌呤放大卵巢黄体细胞中黄体生成激素的作用。
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Hall,AK;Preston,SL;Behrman,HR
通讯作者：
Behrman,HR
影响因子：
4.8
作者：
CONTI, M;KASSON, BG;HSUEH, AJW
通讯作者：
HSUEH, AJW
DOI：
10.1073/pnas.82.2.454
发表时间：
1985-01-01
影响因子：
11.1
作者：
DOWNS, SM;COLEMAN, DL;EPPIG, JJ
通讯作者：
EPPIG, JJ