Isolation and genetic characterization of pGKL killer-insensitive mutants (iki) from Saccharomyces cerevisiae
Isolation and genetic characterization of pGKL killer-insensitive mutants (iki) from Saccharomyces cerevisiae
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DOI：
10.1271/bbb.60.798
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发表时间：
1996-05-01
影响因子：
1.6
通讯作者：
Hishinuma, F
中科院分区：
文献类型：
--
作者：
Kishida, M;Tokunaga, M;Hishinuma, F
线性双链DNA质粒pGKL1编码酵母杀伤毒素复合物（Gunge et al, 1981），其杀伤机制尚不清楚。我们从酿酒酵母中分离并鉴定了8个突变体，这些突变体对细胞内表达的28-kDa杀伤亚基和天然杀伤毒素复合物都不敏感，这些突变（iki1至iki5）均为隐性突变，并将其分为5个互补群。iki2突变被定位到XIII染色体上着丝粒附近的一个位置。我们开发了一种新的筛选系统，从schcharomyces基因文库中分离出iki突变的DNA片段，并分别分离出iki1、iki3和iki4突变的三个DNA片段。
The linear double stranded DNA plasmid pGKL1 encodes the yeast killer toxin complex (Gunge et al., 1981) of which the killing mechanism is not understood, We isolated and characterized eight mutants in Saccharomyces cerevisiae that were insensitive to both the intracellularly expressed 28-kDa killer subunit and the native killer toxin complex, These mutations (iki1 through iki5) were all recessive, and classified into five complementation groups, The iki2 mutation was mapped to a position near the centromere on chromosome XIII. We developed a novel screening system to isolate the DNA fragments complementing the iki mutations from a Sccharomyces gene library, and isolated three DNA fragments that complement the iki1, iki3, and iki4 mutations, respectively.