CHEMICALLY-INDUCED CUTICLE MUTATION AFFECTING EPIDERMAL CONDUCTANCE TO WATER-VAPOR AND DISEASE SUSCEPTIBILITY IN SORGHUM-BICOLOR (L) MOENCH
CHEMICALLY-INDUCED CUTICLE MUTATION AFFECTING EPIDERMAL CONDUCTANCE TO WATER-VAPOR AND DISEASE SUSCEPTIBILITY IN SORGHUM-BICOLOR (L) MOENCH
复制标题
DOI：
10.1104/pp.105.4.1239
复制
发表时间：
1994-08-01
期刊：
影响因子：
7.4
通讯作者：
ASHWORTH, EN
中科院分区：
文献类型：
--
作者：
JENKS, MA;JOLY, RJ;ASHWORTH, EN
对具有改变的表皮蜡（EW）结构的两色高粱无花（BM）突变体的分析发现了一种影响EW和角质层沉积的突变。突变体bm-22的角质层比野生型亲本P954035（WT-P954035）的角质层薄约60%，重量约为野生型亲本P954035（WT-P954035）角质层的五分之一。减少角质层沉积与增加表皮传导水蒸气。EW和角质层沉积的减少增加了对真菌病原体玉米大斑病菌的易感性。有证据表明，这种隐性突变发生在一个单一的基因座与多效性的影响。bm-2、bm-6、bm-22和bm-33的独立发生的基因突变是等位的。这些化学诱导的突变体具有基本相同的EW结构，水分损失和角质层沉积。此外，来自bm-22 x WT-P954035回交的138个F-2植株未显示这些性状的重组。这种近等基因背景下的独特突变为研究植物角质层生物合成、生理和功能提供了一个有用的生物学系统。
Analysis of Sorghum bicolor bloomless (bm) mutants with altered epicuticular wax (EW) structure uncovered a mutation affecting both EW and cuticle deposition. The cuticle of mutant bm-22 was about 60% thinner and approximately one-fifth the weight of the wild-type parent P954035 (WT-P954035) cuticles. Reduced cuticle deposition was associated with increased epidermal conductance to water vapor. The reduction in EW and cuticle deposition increased susceptibility to the fungal pathogen Exserohilum turcicum. Evidence suggests that this recessive mutation occurs at a single locus with pleiotropic effects. The independently occurring gene mutations of bm-2, bm-6, bm-22, and bm-33 are allelic. These chemically induced mutants had essentially identical EW structure, water loss, and cuticle deposition. Furthermore, 138 F-2 plants from a bm-22 x WT-P954035 backcross showed no recombination of these traits. This unique mutation in a near-isogenic background provides a useful biological system to examine plant cuticle biosynthesis, physiology, and function.