Possible involvement of orexin in the stress reaction in rats
Possible involvement of orexin in the stress reaction in rats
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DOI：
10.1006/bbrc.2000.2412
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发表时间：
2000-04-02
影响因子：
3.1
通讯作者：
Murakami, N
中科院分区：
文献类型：
--
作者：
Ida, T;Nakahara, K;Murakami, N
我们研究促肾上腺皮质激素释放因子（CRF）是否参与食欲素诱导的梳理和洗脸行为，以及食欲素是否参与应激反应。α-螺旋CRF，CRF拮抗剂，单独给药，没有行为学影响，但它阻断食欲素诱导的理毛和洗脸行为。皮质酮的水平增加，在剂量依赖性的方式15分钟后，冰冷的注射食欲素，它仍然很高，至少60分钟。在一个月大的大鼠，1小时的固定应激增加食欲素mRNA水平，但不是黑色素浓集激素（MCH）mRNG在外侧下丘脑区（LHA）。在6月龄大鼠中，30分钟的冷应激增加了食欲素mRNA在LHA中的表达。与2个月大的大鼠不同，固定应激没有改变g个月大大鼠的食欲素mRNA表达。这些结果表明，CRF参与食欲素诱导的行为，食欲素可能在一些应激反应中发挥重要作用。(C)北京大学出版社.
We examined whether corticotropin-releasing factor (CRF) was involved in orexin-induced grooming and face-washing behaviors, and whether orexin was involved in the stress reaction. Administration of alpha-helical CRF, CRF antagonist, alone had no behavioral effect, but it blocked the orexin-induced grooming and face-washing behaviors in rats. The level of corticosterone increased in a dose-dependent manner 15 min after icy injection of orexin, and it remained high for at least 60 min. In a-month-old rats, 1 h of immobilization stress increased orexin mRNA levels, but not the melanin-concentrating hormone (MCH) mRNG in the lateral hypothalamic area (LHA). In 6-month-old rats, 30 min of cold stress increased the expression of orexin mRNA in the LHA. Unlike in the 2-month-old rats, immobilization stress did not change orexin mRNA expression in g-month-old rats. These results suggest that CRF is involved in orexin-induced behaviors, and that orexin may play an important role in some stress reactions. (C) 2000 Academic Press.