Unraveling the Phase Behavior, Mechanical Stability, and Protein Reconstitution Properties of Polymer-Lipid Hybrid Vesicles.
Unraveling the Phase Behavior, Mechanical Stability, and Protein Reconstitution Properties of Polymer-Lipid Hybrid Vesicles.
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DOI：
10.1021/acs.biomac.3c00498
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发表时间：
2023-09-11
影响因子：
6.2
通讯作者：
Jeuken, Lars J. C.
中科院分区：
文献类型：
--
作者：
Muller, Wagner A.;Beales, Paul A.;Muniz, Andreï R.;Jeuken, Lars J. C.
由天然磷脂和合成两亲性共聚物组成的混合囊泡已显示出显着的材料特性和生物技术潜力，将聚合物的坚固性与磷脂膜的生物相容性结合起来。为了预测和优化脂质和共聚物的混合行为，以及了解杂化膜和膜蛋白等大分子之间的相互作用，在分子水平上的全面理解是必不可少的。这可以通过分子动力学模拟和实验相结合来实现。在这里，POPC和PBD 22-b-PEO 14杂化膜的模拟显示，揭示不同的共聚物配置取决于聚合物与脂质的比例。高聚合物浓度产生较厚的膜，具有延伸的聚合物构象，而高脂质含量导致聚合物链的崩溃。高浓度的聚合物进一步与降低的面积压缩模量和改变的侧压力曲线相关，假设导致实验观察到的膜蛋白重构和抗去污剂去稳定性的改善。最后，嵌入在双层中的WALP肽的模拟表明，只有聚合物含量高达50%的膜有利于跨膜构型。这些模拟与以前和新的实验结果相关，并提供了更深入的了解脂质共聚物杂化膜的性能。
Hybrid vesicles consisting of natural phospholipids and synthetic amphiphilic copolymers have shown remarkable material properties and potential for biotechnology, combining the robustness of polymers with the biocompatibility of phospholipid membranes. To predict and optimize the mixing behavior of lipids and copolymers, as well as understand the interaction between the hybrid membrane and macromolecules like membrane proteins, a comprehensive understanding at the molecular level is essential. This can be achieved by a combination of molecular dynamics simulations and experiments. Here, simulations of POPC and PBD22-b-PEO14 hybrid membranes are shown, uncovering different copolymer configurations depending on the polymer-to-lipid ratio. High polymer concentrations created thicker membranes with an extended polymer conformation, while high lipid content led to the collapse of the polymer chain. High concentrations of polymer were further correlated with a decreased area compression modulus and altered lateral pressure profiles, hypothesized to result in the experimentally observed improvement in membrane protein reconstitution and resistance toward destabilization by detergents. Finally, simulations of a WALP peptide embedded in the bilayer showed that only membranes with up to 50% polymer content favored a transmembrane configuration. These simulations correlate with previous and new experimental results and provide a deeper understanding of the properties of lipid-copolymer hybrid membranes.
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影响因子：
3.7
作者：
Jing H;Wang Y;Desai PR;Ramamurthi KS;Das S
通讯作者：
Das S
DOI：
10.1016/j.str.2017.03.006
发表时间：
2017-05-02
期刊：
Structure (London, England : 1993)
影响因子：
--
作者：
Aryal P;Jarerattanachat V;Clausen MV;Schewe M;McClenaghan C;Argent L;Conrad LJ;Dong YY;Pike ACW;Carpenter EP;Baukrowitz T;Sansom MSP;Tucker SJ
通讯作者：
Tucker SJ
影响因子：
4.4
作者：
Bereau, Tristan;Bennett, W. F. Drew;Karttunen, Mikko
通讯作者：
Karttunen, Mikko
DOI：
10.1073/pnas.1814775116
发表时间：
2019-03-05
影响因子：
11.1
作者：
Jacobs, Miranda L.;Boyd, Margrethe A.;Kamat, Neha P.
通讯作者：
Kamat, Neha P.
影响因子：
3.4
作者：
Hu, Mingyang;de Jong, Djurre H.;Deserno, Markus
通讯作者：
Deserno, Markus