Aflatoxin B1-4-hydroxylase is associated with cytochrome P3-450 in C57BL/6 mouse liver.
Aflatoxin B1-4-hydroxylase is associated with cytochrome P3-450 in C57BL/6 mouse liver.
复制标题
黄曲霉毒素 B1-4-羟化酶与 C57BL/6 小鼠肝脏中的细胞色素 P3-450 相关。
DOI：
10.1016/0006-291x(87)91494-x
复制
发表时间：
1987
影响因子：
3.1
通讯作者：
Gurtoo,HL
中科院分区：
文献类型：
--
作者：
Koser,PL;Faletto,MB;Bansal,SK;Caballes,L;Bresnick,E;Hines,RN;Gurtoo,HL
来自Aroclor 1254处理的小鼠的肝脏的信使RNA用于产生cDNA文库。通过用大鼠细胞色素P-450 c基因的探针筛选，从该文库中分离出对应于细胞色素P1-450和P3-450的cDNA克隆。从独特的限制性片段制备P1-450和P3-450的特异性非交叉杂交探针。将放射性标记的探针与来自用低（15 mg/kg）和高（150 mg/kg，400 mg/kg）剂量的β-萘甲酮处理的小鼠的RNA杂交。低剂量的β-萘酚酮仅诱导P3-450 mRNA的表达，而高剂量的β-萘酚酮诱导P1-450和P3-450 mRNA的表达。同样，低剂量的β-萘甲酮诱导黄曲霉毒素B1-4-羟化酶，而较高剂量的β-萘甲酮诱导黄曲霉毒素B1-4-羟化酶和芳烃羟化酶的活性。这些结果表明，P3-450 mRNA编码与黄曲霉毒素B1-4-羟化酶活性相关的细胞色素。
Messenger RNA from the livers of Aroclor 1254 treated mice was used to produce a cDNA library. cDNA clones corresponding to cytochromes P1-450 and P3-450 were isolated from this library by screening with a probe for the rat cytochrome P-450c gene. Specific non-cross hybridizing probes for P1-450 and P3-450 were prepared from unique restriction fragments. The radio-labeled probes were hybridized to RNA from mice treated with a low (15 mg/kg) and high (150 mg/kg, 400 mg/kg) doses of β-naphthoflavone. The low dose of β-naphthoflavone was found to induce only P3-450 mRNA, whereas higher doses induced both P1-450 and P3-450 mRNA. Similarly, a low dose of β-naphthoflavone induced aflatoxin B1-4-hydroxylase, whereas higher doses induced both aflatoxin B1-4-hydroxylase and aryl hydrocarbon hydroxylase activities. These results suggest that P3-450 mRNA codes for the cytochrome that is associated with aflatoxin B1-4-hydroxylase activity.