A red emitting fluorescent probe for imaging mitochondrial hydrogen polysulfide in living cells and tissues
A red emitting fluorescent probe for imaging mitochondrial hydrogen polysulfide in living cells and tissues
复制标题
用于活细胞和组织中线粒体多硫化氢成像的红色发射荧光探针
DOI：
10.1016/j.snb.2018.10.155
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发表时间：
2019-02-15
影响因子：
8.4
通讯作者：
Xiao, Kai
中科院分区：
文献类型：
--
作者：
Meng, Wenqi;Shi, Wenwen;Xiao, Kai
多硫化氢（H2Sn）引起了越来越多的关注，因为H2Sn代替H2S已被证明是生理和病理过程中的实际信号分子。因此，生物科学迫切需要开发准确、灵敏的 H2Sn 检测方法。在这项工作中，我们开发了一种红色发射线粒体靶向荧光探针 HQO-PSP，它能够跟踪 H2Sn。 HQO-PSP具有低细胞毒性、良好的选择性和高灵敏度，能够检测活细胞中的H2Sn。此外，我们还证明 HQO-PSP 也可以成功应用于识别活体组织中的 H2Sn。
Hydrogen polysulfide (H2Sn) has attracted increasing attention since H2Sn instead of H2S has been shown to be the actual signaling molecule in physiological and pathological processes. Thus, developing accurate and sensitive assays for H2Sn detection is urgently needed in biological science. In this work, we developed a red emitting mitochondria-targeting fluorescent probe, HQO-PSP, that is capable of tracking H2Sn. With low cytotoxicity, good selectively and high sensitively, HQO-PSP was able to detect H2Sn in living cells. Furthermore, we also demonstrated that HQO-PSP could also be successfully applied to recognize H2Sn in living tissues.