Estimating accuracy of RNA-Seq and microarrays with proteomics
Estimating accuracy of RNA-Seq and microarrays with proteomics
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DOI：
10.1186/1471-2164-10-161
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发表时间：
2009-04-16
期刊：
影响因子：
4.4
通讯作者：
Khaitovich, Philipp
中科院分区：
文献类型：
--
作者：
Fu, Xing;Fu, Ning;Khaitovich, Philipp
背景：微阵列通过在转录组范围内进行基因表达比较，彻底改变了生物学研究。然而，微阵列不能准确估计绝对表达水平。目前，高通量测序正在成为转录组研究的替代方法。虽然免费的许多限制所施加的微阵列设计，其潜力估计绝对转录水平是unknown.Results：在这项研究中，我们评估相对准确性的微阵列和转录组测序（RNA-Seq）使用第三种方法：蛋白质组学。我们发现，RNA-Seq提供了一个更好的估计绝对expression levels.Conclusion：我们的研究结果表明，在整体技术性能方面，RNA-Seq是需要准确估计绝对转录水平的研究的首选技术。
Background: Microarrays revolutionized biological research by enabling gene expression comparisons on a transcriptome-wide scale. Microarrays, however, do not estimate absolute expression level accurately. At present, high throughput sequencing is emerging as an alternative methodology for transcriptome studies. Although free of many limitations imposed by microarray design, its potential to estimate absolute transcript levels is unknown.Results: In this study, we evaluate relative accuracy of microarrays and transcriptome sequencing (RNA-Seq) using third methodology: proteomics. We find that RNA-Seq provides a better estimate of absolute expression levels.Conclusion: Our result shows that in terms of overall technical performance, RNA-Seq is the technique of choice for studies that require accurate estimation of absolute transcript levels.