Transcriptomic and proteomic analyses of splenic immune mechanisms of rainbow trout (Oncorhynchus mykiss) infected by Aeromonas salmonicida subsp salmonicida
Transcriptomic and proteomic analyses of splenic immune mechanisms of rainbow trout (Oncorhynchus mykiss) infected by Aeromonas salmonicida subsp salmonicida
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DOI：
10.1016/j.jprot.2015.03.031
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发表时间：
2015-06-03
影响因子：
3.3
通讯作者：
Li, Aihua
中科院分区：
文献类型：
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作者：
Long, Meng;Zhao, Juan;Li, Aihua
由杀鲑气单胞菌杀鲑亚种引起的疖病。杀鲑病是包括虹鳟鱼（Oncorhynchusmykiss）在内的鲑科鱼类中的流行病。然而，虹鳟鱼对A.杀鲑鱼的生物还没有被完全了解。本研究以虹鳟鱼为材料，在感染A.通过使用Illumina-seq和iTRAQ方法，分别在转录组和蛋白质组水平上对杀鲑素进行分析。结果表明，在脾脏免疫应答过程中，共有1036个基因和133个蛋白质发生了差异表达。沙门氏菌感染对差异表达的基因和蛋白质进行基因本体和KEGG分析，发现免疫系统过程和对刺激的反应是前两大生物学过程，免疫系统、信号分子和相互作用以及免疫疾病是激活的差异通路。转录组学和蛋白质组学结果的相关性分析显示，17个蛋白质（11个上调和6个下调）在RNA和蛋白质水平具有一致的表达。此外，蛋白质相互作用分析表明，疾病，蛋白酶体，氨酰-tRNA的生物合成和核苷酸代谢之间的一致表达的蛋白质的主要相互作用。因此，这些上调的蛋白质，即铁蛋白、CD 209、IL 13 R α 1、VDAC 2、GIMAP 7、PSMA 1、本研究通过对虹鳟鱼脾脏中ANXA 11和ANXA 11基因及其相应蛋白产物的差异表达分析，首次发现了虹鳟鱼免疫反应的生物标志物。感染A。杀鲑素，揭示了虹鳟鱼对A.为鱼类免疫学研究提供了新的分子靶点。(C)2015 Elsevier B. V.版权所有。
Furunculosis caused by Aeromonas salmonicida subsp. salmonicida is an epidemic disease among salmonids, including rainbow trout (Oncorhynchus mykiss). However, the immune mechanisms that are elicited in rainbow trout against the invasion of A. salmonicida are not yet fully understood. In this study, we examined the spleen to investigate the immune response of rainbow trout at 3 days post-infection by A. salmonicida at the transcriptome and proteome levels by using Illumina-seq and iTRAQ methods, respectively. A total of 1036 genes and 133 proteins were found to undergo differential expression during the immune response of the spleen against A. salmonicida infection. Gene ontology and KEGG analysis were conducted among the differentially expressed genes and proteins, revealing that immune system process and response to stimulus were the top two biological processes, and immune system, signaling molecules and interaction, and immune diseases were the differential pathways activated. Correlation analysis of transcriptomic and proteomic results showed 17 proteins (11 upregulated and 6 downregulated) having consistent expression at RNA and protein levels. Moreover, protein protein interaction analysis showed that diseases, proteasome, aminoacyl-tRNA biosynthesis, and nucleotide metabolism were the main interactions among the consistently expressed proteins. Consequently, these upregulated proteins, namely, ferritin, CD209, IL13R alpha 1, VDAC2, GIMAP7, PSMA1, and two ANXA11s could be considered as potential biomarkers for rainbow trout immune responses.Biological significanceThis study provides the first identification of immune markers through an analysis of the differential expression of both genes and their corresponding protein products in the spleen of rainbow trout after infection by A. salmonicida, shedding light on the molecular mechanisms triggered in rainbow trout against A. salmonicida infection and providing new molecular targets for further immunological research in fish. (C) 2015 Elsevier B.V. All rights reserved.