DNA Detection on a Power-free Microchip with Laminar Flow-assisted Dendritic Amplification
DNA Detection on a Power-free Microchip with Laminar Flow-assisted Dendritic Amplification
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DOI：
10.2116/analsci.26.1053
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发表时间：
2010-10-01
影响因子：
1.6
通讯作者：
Maeda, Mizuo
中科院分区：
文献类型：
--
作者：
Hosokawa, Kazuo;Sato, Takahiro;Maeda, Mizuo
在本文中，我们描述了我们的两种原始技术，无功率微芯片和层流辅助树突状扩增（LFDA）的DNA检测实验。将具有微通道图案的聚二甲基硅氧烷（PDMS）部件与探针DNA修饰的玻璃板结合制成微芯片，进行了21碱基生物素化靶DNA检测和56碱基未标记靶DNA与生物素化探针DNA夹心杂交检测两种实验。杂交反应后，将fitc标记的链霉亲和素和生物素化的抗链霉亲和素抗体注射到反应部位，开始LFDA，检测时间为20 mm，生物素化目标的检测限（LOD）为22 2pm， SNP的检测限（LOD）为10 - 30 pM。取决于SNP类型。
In this paper, we describe DNA detection experiments using our two original technologies, power-free microchip and laminar flow-assisted dendritic amplification (LFDA). which were previously applied to immunoassays A microchip was fabricated by combining a poly(dimethylsiloxane) (PDMS) part having microchannel patterns and a glass plate modified with probe DNA We carried out two kinds of experiments the detection of 21-base biotinylated target DNA and the detection of single-nucleotide polymorphism (SNP) in 56-base unlabeled target DNA by sandwich hybridization with biotinylated probe DNA For both of the experiments, the necessary solutions were injected into microchannels not by an external power source, but by air dissolution into the PDMS part After a hybridization reaction, the LFDA was started by injecting FITC-labeled streptavidin and biotinylated anti-streptavidin antibody onto the reaction site With a detection time of 20 mm. the limit of detection (LOD) for the biotinylated target was 2 2 pM, and the LOD for the SNP was 10 - 30 pM. depending on the SNP type.