Improved binding site assignment by high-resolution mapping of RNA-protein interactions using iCLIP.
Improved binding site assignment by high-resolution mapping of RNA-protein interactions using iCLIP.
复制标题
DOI：
10.1038/ncomms8921
复制
发表时间：
2015-08-11
影响因子：
16.6
通讯作者：
Kulozik AE
中科院分区：
文献类型：
--
作者：
Hauer C;Curk T;Anders S;Schwarzl T;Alleaume AM;Sieber J;Hollerer I;Bhuvanagiri M;Huber W;Hentze MW;Kulozik AE
Individual-nucleotide resolution crosslinking and immunoprecipitation (iCLIP) allows the determination of crosslinking sites of RNA-binding proteins (RBPs) on RNAs. iCLIP is based on ultraviolet light crosslinking of RBPs to RNA, reverse transcription and high-throughput sequencing of fragments terminating at the site of crosslinking. As a result, start sites of iCLIP fragments are expected to cluster with a narrow distribution, typically representing the site of direct interaction between the RBP and the RNA. Here we show that for several RBPs (eIF4A3, PTB, SRSF3, SRSF4 and hnRNP L), the start sites of iCLIP fragments show a fragment length-dependent broader distribution that can be shifted to positions upstream of the known RNA-binding site. We developed an analysis tool that identifies these shifts and can improve the positioning of RBP binding sites. Individual-nucleotide resolution crosslinking and immunoprecipitation (iCLIP) can map RNA binding sites of RNA-binding proteins (RBPs). Here, the authors report an analysis tool that improves the binding site assignment for some RBPs that have length-dependent broader distribution for their iCLIP fragments.
