MmfL catalyses formation of a phosphorylated butenolide intermediate in methylenomycin furan biosynthesis.
MmfL catalyses formation of a phosphorylated butenolide intermediate in methylenomycin furan biosynthesis.
复制标题
DOI：
10.1039/d0cc05658h
复制
发表时间：
2020-11
影响因子：
4.9
通讯作者：
Shanshan Zhou;Nicolas Malet;Lijiang Song;C. Corre;G. Challis
中科院分区：
文献类型：
--
作者：
Shanshan Zhou;Nicolas Malet;Lijiang Song;C. Corre;G. Challis
使用合成底物类似物和产品标准的组合，γ-丁内酯生物合成酶AFSA的同系物MmfL被证明催化二羟丙酮磷酸与β-酮酰基硫酸酯的缩合，形成亚甲基霉素呋喃生物合成中的磷酸化丁烯内酯中间体，从而诱导天蓝色链霉菌A3(2)产生亚甲氧霉素抗生素。AFSA同系物也参与了其他链霉菌中2-烷基-4-羟基-3-甲基丁烯内酯的生物合成，表明不同的信号分子是由类似的磷酸化丁烯内酯中间体组装而成的。
Using a combination of a synthetic substrate analogue and product standard, MmfL, a homologue of the γ-butyrolactone biosynthetic enzyme AfsA, was shown to catalyse the condensation of dihydroxyacetone phosphate with a β-ketoacyl thioester to form a phosphorylated butenolide intermediate in the biosynthesis of the methylenomycin furans, which induce methlenomycin antibiotic production in Streptomyces coelicolor A3(2). AfsA homologues are also involved in the biosynthesis of 2-akyl-4-hydroxy-3-methyl butenolide inducers of antibiotic production in other Streptomyces species, indicating that diverse signalling molecules are assembled from analogous phosphorylated butenolide intermediates.