Natural variation in the temperature range permissive for vernalization in accessions of Arabidopsis thaliana.
Natural variation in the temperature range permissive for vernalization in accessions of Arabidopsis thaliana.
复制标题
拟南芥种质春化允许的温度范围的自然变化。
DOI：
10.1111/j.1365-3040.2012.02548.x
复制
发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Amasino,RichardM
中科院分区：
文献类型：
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作者：
Wollenberg,AmandaC;Amasino,RichardM
春化作用是对寒冷的反应，通常在实验室中在接近冰点（2-4 °C）的温度下进行研究。 许多需要春化的物种，如拟南芥，在一系列具有不同冬季温度的栖息地中被发现。在自然温度范围内的变化，激发了春化反应inopsishas还没有得到充分的探讨。我们描述了中间温度（7-19 °C）对15份材料和研究充分的参考系Col‐FRI的影响。 虽然逐渐升高的温度在激活春化特异性基因VERNALIZATION-INSENSITIVE 3（VIN 3）的表达和加速开花方面的效果逐渐降低，但开花时间响应的上限（Tmax）存在显著的自然变化。VIN 3是Col-FRI的Tmax（13 °C）响应所必需的。 令人惊讶的是，即使16 °C处理也能在6个测试品系中诱导VIN 3，尽管该温度对加速其中两个品系的开花无效。 最后，我们提出的证据表明，在至少一个加入中，通过19 °C暴露适度加速开花可能会抵消由非诱导光周期引起的开花时间延迟，从而产生光周期不敏感的外观。
Vernalization is an acceleration of flowering in response to chilling, and is normally studied in the laboratory at near‐freezing (2–4 °C) temperatures. Many vernalization‐requiring species, such asArabidopsis thaliana, are found in a range of habitats with varying winter temperatures. Natural variation in the temperature range that elicits a vernalization response inArabidopsishas not been fully explored. We characterized the effect of intermediate temperatures (7–19 °C) on 15 accessions and the well‐studied reference line Col‐FRI. Although progressively warmer temperatures are gradually less effective at activating expression of the vernalization‐specific geneVERNALIZATION‐INSENSITIVE 3(VIN3) and in accelerating flowering, there is substantial natural variation in the upper threshold (Tmax) of the flowering‐time response.VIN3is required for theTmax(13 °C) response of Col‐FRI. Surprisingly, even 16 °C treatment caused induction ofVIN3in six tested lines, despite the ineffectiveness of this temperature in accelerating flowering for two of them. Finally, we present evidence that mild acceleration of flowering by 19 °C exposure may counterbalance the flowering time delay caused by non‐inductive photoperiods in at least one accession, creating an appearance of photoperiod insensitivity.