Selective autophagy: Lysophagy
Selective autophagy: Lysophagy
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DOI：
10.1016/j.ymeth.2014.12.014
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发表时间：
2015-03-15
期刊：
影响因子：
4.8
通讯作者：
Yoshimori, Tamotsu
中科院分区：
文献类型：
--
作者：
Hasegawa, Junya;Maejima, Ikuko;Yoshimori, Tamotsu
自噬是一种在营养缺乏等应激条件下诱导的大量降解系统。选择性自噬，包括异种自噬和线粒体自噬，被认为在多种疾病的发生发展中起重要作用。因此，选择性自噬是一个潜在的治疗靶点。最近的工作表明，溶酶体，一种膜结合的酸性细胞器，当其膜损伤时，通过自噬选择性地隔离;这种现象称为“噬菌性”。溶酶体可被多种原因损伤，包括淀粉样蛋白和矿物晶体，如二氧化硅和尿酸盐，这将引发神经变性和其他疾病。在本节中，我们提供了用于监测哺乳动物培养细胞中的噬血作用的方法的概述。这些方法可用于评估目标分子参与选择性自噬的情况，以及用于筛选参与选择性自噬的新型蛋白质。(C)2014 Elsevier Inc. All rights reserved.
Autophagy is a bulk degradation system that is induced under stress conditions such as nutrient deprivation. Selective autophagy, including xenophagy and mitophagy, is believed to play important roles in the development of several diseases. Consequently, selective autophagy represents a potential therapeutic target. Recent work showed that the lysosome, a membrane-bound acidic organelle, is selectively sequestered by autophagy when its membrane is injured; this phenomenon is called "lysophagy". Lysosomes can be injured by diverse causes, including amyloid proteins and mineral crystals such as silica and monosodium urate, which would trigger neurodegeneration and other diseases. In this section, we provide an overview of methods for monitoring lysophagy in mammalian cultured cells. These methods can be used to evaluate the involvement of molecules of interest in selective autophagy, and in screens aimed at identifying novel proteins engaged in selective autophagy. (C) 2014 Elsevier Inc. All rights reserved.