An in vivo rat model for visualizing glioma tumor cell invasion using stable persistent expression of the green fluorescent protein.
An in vivo rat model for visualizing glioma tumor cell invasion using stable persistent expression of the green fluorescent protein.
复制标题
使用绿色荧光蛋白的稳定持续表达来可视化神经胶质瘤肿瘤细胞侵袭的体内大鼠模型。
DOI：
10.1016/s0304-3835(99)00053-1
复制
发表时间：
1999
期刊：
影响因子：
9.7
通讯作者：
Broaddus,WC
中科院分区：
文献类型：
--
作者：
Fillmore,HL;Shurm,J;Furqueron,P;Prabhu,SS;Gillies,GT;Broaddus,WC
在我们研究脑肿瘤细胞迁移/侵袭宿主组织的机制的研究中，利用绿色荧光蛋白(GFP)DNA构建稳定地转染了RT2大鼠胶质瘤细胞系。稳定转染的RT2细胞在最初传代时表现出高GFP表达，并在30代以上保持表达，并且与对照组相比具有相似的体外和体内生长模式。在大鼠脑组织中，可以在不进行任何后处理的情况下检测到单个肿瘤细胞。利用流式细胞术和细胞分选技术，我们能够从荷瘤动物的肿瘤核心以及邻近组织和对侧大脑半球中提取和培养GFP阳性细胞。
In our studies examining mechanisms of brain tumor cell migration/invasion into host tissue, the RT2 rat glioma cell line was stably transfected utilizing a green fluorescent protein (GFP) DNA construct. Stable transfected RT2 cells demonstrate high GFP expression at initial passages, maintain expression over 30 passages and have similar in vitro and in vivo growth patterns relative to controls. In rat brain tissue, individual tumor cells can be detected without any post-treatment. Using flow cytometry and cell sorting, we are able to retrieve and culture GFP positive cells from tumor core as well as adjacent tissue and contralateral hemisphere of tumor-bearing animals.