Eimeria tenella rhomboid 3 has a potential role in microneme protein cleavage.
Eimeria tenella rhomboid 3 has a potential role in microneme protein cleavage.
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Eimeria tenella rhomboid 3 在微线体蛋白裂解中具有潜在作用。
DOI：
10.1016/j.vetpar.2014.01.010
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发表时间：
2014-03
影响因子：
2.6
通讯作者：
Jianhua Li
中科院分区：
文献类型：
--
作者：
Jun Zheng;Pengtao Gong;Honglin Jia;Mingying Li;Guocai Zhang;Xichen Zhang;Jianhua Li
包括柔弱美耳美球虫在内的几种顶复合体寄生虫的入侵伴随着菱形蛋白酶介导的膜内蛋白水解导致表面粘附素的脱落。我们之前已经确认过了。tenellarhomboid 3 (etrm3)，但其确切作用尚未阐明。本研究利用酵母双杂交技术分析了EtROM3与miceme蛋白之间的相互作用。结果表明，在共转化的AH109酵母中，c-Myc-ROM3融合蛋白与EtMIC4蛋白相互作用，免疫沉淀法进一步证实了这一点。共转化细胞的EtMIC4条带较小，表明etrm3是一种活性蛋白酶，参与了EtMIC4的裂解。
Invasion in several apicomplexan parasites, includingEimeria tenella, is accompanied by shedding of surface adhesins by intramembrane proteolysis mediated by rhomboid protease. We have previously identifiedE. tenellarhomboid 3 (EtROM3), but its precise role has not been elucidated. In this study, the interactions between EtROM3 and microneme (MIC) proteins were analyzed using the yeast two hybrid technique. The results showed that c-Myc-ROM3 fusion protein interacted with EtMIC4 protein in co-transformed AH109 yeasts, which was further confirmed by immunoprecipitation assay. Smaller EtMIC4 band from co-transformed cells suggested that EtROM3 was an active protease and involved in the cleavage of EtMIC4.
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影响因子：
6.7
作者：
Baker RP;Wijetilaka R;Urban S
通讯作者：
Urban S
DOI：
10.1042/0264-6021:3630347
发表时间：
2002-04
期刊：
The Biochemical journal
影响因子：
--
作者：
J. Pascall;J. Luck;K. Brown
通讯作者：
J. Pascall;J. Luck;K. Brown
影响因子：
6.7
作者：
Buguliskis JS;Brossier F;Shuman J;Sibley LD
通讯作者：
Sibley LD
DOI：
10.1073/pnas.0407918102
发表时间：
2005-03-15
影响因子：
11.1
作者：
Brossier, F;Jewett, TJ;Urban, S
通讯作者：
Urban, S
影响因子：
7.8
作者：
O'Donnell, Rebecca A;Hackett, Fiona;Howell, Steven A;Treeck, Moritz;Struck, Nicole;Krnajski, Zita;Withers-Martinez, Chrislaine;Gilberger, Tim W;Blackman, Michael J
通讯作者：
Blackman, Michael J