Interleukin-6 enhances the fertilizing capacity of human sperm by increasing capacitation and acrosome reaction.
Interleukin-6 enhances the fertilizing capacity of human sperm by increasing capacitation and acrosome reaction.
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DOI：
10.1002/j.1939-4640.1994.tb00438.x
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发表时间：
1994-05
影响因子：
--
通讯作者：
Rajesh K. Naz;Paul Kaplan
中科院分区：
文献类型：
--
作者：
Rajesh K. Naz;Paul Kaplan
研究了重组人白细胞介素-6（IL-6）对人精子穿透去透明带仓鼠卵（SPA）、人精子获能和顶体反应的影响。高浓度（60-600 pg/100 μ l）的IL-6对人精子在SPA中的穿透率有显著的促进作用（P < 0.001）。这种作用是特异性的，因为用特异性中和抗体进行免疫吸附完全消除了IL-6的增强作用。在相同浓度下，IL-6还显著增强人精子细胞自发的顶体反应和顶体酶的释放，以及钙离子载体诱导的顶体反应。IL-6对精子活力百分比没有影响，但它显著影响各种运动特性，如精子细胞的速度、线性、侧向头部位移（ALH）幅度和搏动频率--这些运动参数涉及精子细胞的超活化现象。这些结果首次证明IL-6通过增加获能导致顶体反应而在增强人类精子的获能能力中提供了阳性信号，因此其可能在人类男性不育症的治疗中具有临床应用。
The effects of human recombinant interleukin-6 (IL-6) on human sperm penetration of zona-free hamster ova (SPA) and human sperm cell capacitation and acrosome reaction were investigated. IL-6 at higher concentrations (60-600 pg/100 microliters) significantly (P < 0.001) increased rather than decreased the human sperm penetration rates in SPA. The effects were specific because immunoadsorption with the specific neutralizing antibody completely abolished the enhancing effects of IL-6. At the same concentrations, IL-6 also significantly enhanced spontaneous as well as calcium ionophore-induced acrosome reaction and release of acrosin from the human sperm cells. There was no effect of IL-6 on percent sperm motility, although it significantly affected various motility characteristics such as velocity, linearity, amplitude of lateral head displacement (ALH), and beat frequency of sperm cells--the motility parameters involved in hyperactivation phenomenon of sperm cells. These results demonstrate for the first time that IL-6 provides a positive signal in enhancing fertilizing capacity of human sperm by increasing capacitation leading to acrosome reaction, and thus it may have clinical applications in the treatment of male infertility in humans.