EFFECTS OF AFLATOXIN B-1 ON MURINE LYMPHOCYTIC FUNCTIONS
EFFECTS OF AFLATOXIN B-1 ON MURINE LYMPHOCYTIC FUNCTIONS
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DOI：
10.1016/0300-483x(89)90076-0
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发表时间：
1989-01-01
期刊：
影响因子：
4.5
通讯作者：
SHARMA, RP
中科院分区：
文献类型：
--
作者：
REDDY, RV;SHARMA, RP
我们最近报道了黄曲霉毒素B1 （AFB1）对小鼠的免疫毒性作用，黄曲霉毒素B1是黄曲霉的次生真菌代谢产物。本文描述了AFB1在体内或体外暴露后对淋巴细胞功能的影响。雄性BALB/c小鼠分别给予0、0.03、0.145或0.7 mg/kg体重的AFB1治疗4周。在有丝分裂原（脂多糖、植物血凝素、美洲商陆有丝分裂原和刀豆蛋白A）的淋巴细胞培养中，观察到DNA合成的剂量相关性下降。未观察到对蛋白质或核糖核酸合成的影响。外周血白细胞计数和自然杀伤细胞功能呈剂量依赖性下降。用10-6 ~ 10-4 M AFB1培养的正常小鼠脾淋巴细胞DNA合成降低了100倍。10-5 M时，10-4 M时蛋白质合成减少，10-4 M时RNA合成减少2.5倍。10-5 m，体外添加AFB1 （bbb50 .mu）。M)减少了豆豆蛋白a诱导的抑制细胞的产生。结果表明，AFB1对淋巴细胞具有直接和复杂的作用，不同亚群的敏感性存在差异。
We recently reported on the immunotoxic effects of Aflatoxin B1 (AFB1), a secondary fungal metabolite of Aspergillus flavus in mice. The present paper describes the effect of AFB1 on cellular functions in lymphocytes after in vivo or in vitro exposures. Male BALB/c mice received 0, 0.03, 0.145 or 0.7 mg/kg body weight of AFB1 for 4 weeks. A dose-related decrease in DNA synthesis in lymphocyte cultures with or without the mitogens (lipopolysaccharide, phytohemagglutinin, pokeweed mitogen and concanavalin A) was observed. No effects on protein or ribonucleic acid synthesis were observed. There were dose-dependent decreases in peripheral leucocyte counts and natural killer cell function. Splenic lymphocytes from normal mice cultured with 10-6 to 10-4 M AFB1 had decreased DNA synthesis at > 1 .times. 10-5 M, a decreased protein synthesis at 10-4 M and decreased RNA synthesis at > 2.5 .times. 10-5 M. In vitro addition of AFB1, (> 50 .mu.M) reduced generation of concanavalin A-induced suppressor cells. The results suggest that AFB1 had a direct and complex effect on lymphocytes and there is a differential sensitivity of various subpopulations.