Anchoring structure of the calvarial periosteum revealed by focused ion beam/scanning electron microscope tomography.
Anchoring structure of the calvarial periosteum revealed by focused ion beam/scanning electron microscope tomography.
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DOI：
10.1038/srep17511
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发表时间：
2015-12-02
影响因子：
4.6
通讯作者：
Nakamura K
中科院分区：
文献类型：
--
作者：
Hirashima S;Ohta K;Kanazawa T;Uemura K;Togo A;Yoshitomi M;Okayama S;Kusukawa J;Nakamura K
再生疗法的一个重要考虑因素是器官周围的组织支持其功能。了解骨膜的结构有助于更有效的骨再生治疗。作为一种细胞来源，骨膜还有助于骨骼生长和骨折愈合；这进一步需要它与骨骼直接接触。然而，它的锚定强度似乎出人意料地强于预期。在这项研究中，我们使用聚焦离子束/扫描电子显微镜断层扫描来研究超薄的连续切片以及骨膜的三维超微结构，以阐明其锚定强度的结构，因为这种评估使用传统方法是具有挑战性的。我们发现穿孔纤维从骨骼表面以30度角出现。此外，横跨成骨细胞层的纤维经常相互连接，形成网状结构。可观察到成纤维细胞突起延伸至穿孔纤维，其形态与典型的成纤维细胞不同。因此，我们的研究揭示了支持锚定的骨膜的新的超微结构，并作为细胞来源和机械应力传递。
An important consideration in regeneration therapy is the fact that the tissue surrounding an organ supports its function. Understanding the structure of the periosteum can contribute to more effective bone regeneration therapy. As a cellular source, the periosteum also assists bone growth and fracture healing; this further necessitates its direct contact with the bone. However, its anchoring strength appears to be inexplicably stronger than expected. In this study, we used focused ion beam/scanning electron microscope tomography to investigate ultrathin serial sections as well as the three dimensional ultrastructure of the periosteum to clarify the architecture of its anchoring strength, as such assessments are challenging using conventional methods. We discovered perforating fibres that arise from the bone surface at 30 degree angles. Additionally, the fibres across the osteoblast layer were frequently interconnected to form a net-like structure. Fibroblast processes were observed extending into the perforating fibres; their morphologies were distinct from those of typical fibroblasts. Thus, our study revealed novel ultrastructures of the periosteum that support anchorage and serve as a cellular source as well as a mechanical stress transmitter.