Sex- and tissue-specific expression of a cytochrome P450 2C2-luciferase transgene.
Sex- and tissue-specific expression of a cytochrome P450 2C2-luciferase transgene.
复制标题
细胞色素 P450 2C2-荧光素酶转基因的性别和组织特异性表达。
DOI：
10.1016/0303-7207(96)03823-3
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发表时间：
1996
影响因子：
4.1
通讯作者：
Kemper,B
中科院分区：
文献类型：
--
作者：
Li,H;Liu,S;Kemper,B
为了研究兔细胞色素 P450 2C2 基因 (CYP2C2) 的转录调控，使用含有与荧光素酶报告基因融合的 CYP2C2 5' 侧翼区域的 DNA 片段生成转基因小鼠。在三个转基因品系之一中，该转基因在肝脏中表达，并在肾脏中表达较低水平，类似于该基因在兔子中的组织特异性模式。与兔子中的表达不同，转基因在肝脏和肾脏以及两性的大脑中以男性特异性模式表达。雄性小鼠中转基因的表达在去势后受到抑制，而雌性小鼠中的表达在睾酮治疗和定期注射大鼠生长激素（GH）后受到刺激。该转基因的表达也在肝脏中受到发育调节。这些结果表明，CYP2C2 5'侧翼区的 3500 bp 包含组织特异性和发育表达的基础转录调控元件。尽管不能排除位置效应，但该转基因也可能包含用于性二态性表达的顺式调节元件。
To study the transcriptional regulation of the rabbit cytochrome P450 2C2 gene (CYP2C2), transgenic mice were generated using a DNA fragment containing the CYP2C2 5′-flanking region fused to a luciferase reporter gene. In one of three transgenic lines, this transgene was expressed in the liver and at a lower level in the kidney, similar to the tissue-specific pattern of this gene in rabbits. Unlike expression in rabbits, the transgene was expressed in a male-specific pattern in liver and kidney, and in the brain of both sexes. Expression of the transgene in male mice was repressed after castration, while expression in females was stimulated after testosterone treatment and periodic injection of rat growth hormone (GH). The expression of this transgene was also regulated developmentally in the liver. These results suggest that this 3500 bp of CYP2C2 5′-flanking region contains the basal transcriptional regulation elements for tissue-specific and developmental expression. Although a position effect can not be ruled out, this transgene may also include the cis-regulatory element for sexual dimorphic expression.