The omega sequence of VirD2 is important but not essential for efficient transfer of T-DNA by Agrobacterium tumefaciens
The omega sequence of VirD2 is important but not essential for efficient transfer of T-DNA by Agrobacterium tumefaciens
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DOI：
10.1094/mpmi.1998.11.1.57
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发表时间：
1998-01-01
影响因子：
3.5
通讯作者：
Tinland, B
中科院分区：
文献类型：
--
作者：
Bravo-Angel, AM;Hohn, B;Tinland, B
根癌农杆菌的VirD 2蛋白含有在植物细胞转化期间加工和转移T-DNA所必需的确定序列。我们进行了一个保守的VirD 2，其作用已被证明是难以阐明的omega序列的突变分析，到目前为止，在这份报告中，我们表明，这5个氨基酸的缺失或其替换为5个甘氨酸减少T-DNA转移相当大，与野生型相比，表明欧米茄序列是重要的T-DNA的有效转移。然而，T-DNA整合的效率和模式不受Ω序列的任何修饰的影响。讨论了VirD 2的C端对T-DNA转移的重要性。
The VirD2 protein of Agrobacterium tumefaciens contains defined sequences necessary for processing and transferring the T-DNA during transformation of plant cells. We performed a mutational analysis of the conserved omega sequence of VirD2, whose role has proven to be difficult to elucidate so far, In this report, we show that a deletion of these 5 amino acids or their replacement by 5 glycines reduced T-DNA transfer considerably, compared with wild type, demonstrating that the omega sequence is important for the efficient transfer of T-DNAs. However, the efficiency and pattern of integration of the T-DNAs were not affected by any modifications of the omega sequence. The importance of the C terminus of VirD2 for T-DNA transfer is discussed.