Automated resolution independent method for comparing in vivo and dry trabecular bone
Automated resolution independent method for comparing in vivo and dry trabecular bone
复制标题
用于比较体内骨小梁和干燥骨小梁的自动分辨率独立方法
DOI：
10.1002/ajpa.24181
复制
发表时间：
2020
影响因子：
2.8
通讯作者：
Stock, Jay T.
中科院分区：
文献类型：
--
作者：
Saers, Jaap P. P.;DeMars, Lily J.;Stephens, Nicholas B.;Jashashvili, Tea;Carlson, Kristian J.;Gordon, Adam D.;Ryan, Timothy M.;Stock, Jay T.
目的人类骨小梁形态的变异与习惯性行为有关，但由于需要高分辨率的辐射，在体内进行研究是困难的。因此，小梁形态的功能解释仍然是推论性的。在这里，我们介绍了一种方法来连接低和高分辨率的CT数据从干骨和新鲜骨，使骨功能适应在体内进行研究，结果相比，化石和考古record.Materials和methodsWe检查51人干骨远端tibrings从尼罗河谷和英国和两个猪tibrings含有软组织。我们在4%骨长度的同源单层切片中比较了低分辨率外周定量计算机断层扫描（pQCT）参数和高分辨率微CT（μCT），并将结果与我们的新骨比预测（BRP）方法进行了比较。结果低分辨率pQCT图像的线性衰减系数与高分辨率μCT扫描的骨面积/总面积（BA/TA）之间的回归斜率在地理子样本之间存在显著差异，可能是由于成岩作用。BRP准确预测BA/TA（R2= .97），并消除了地理聚类。BRP可以准确估计含有软组织的猪的BA/TA（R2= 0.98），而不需要了解扫描的真密度或体模校准。DiscussionBRP允许在考古骨和湿标本中使用不同能量设置自动比较来自不同图像模式（pQCT，μCT）的图像数据。该方法能够将体内生成的低分辨率数据与化石和考古记录进行比较。这种实验方法将大大改善基于骨小梁微观结构的行为推断。
ObjectivesVariation in human trabecular bone morphology can be linked to habitual behavior, but it is difficult to investigate in vivo due to the radiation required at high resolution. Consequently, functional interpretations of trabecular morphology remain inferential. Here we introduce a method to link low‐ and high‐resolution CT data from dry and fresh bone, enabling bone functional adaptation to be studied in vivo and results compared to the fossil and archaeological record.Materials and methodsWe examine 51 human dry bone distal tibiae from Nile Valley and UK and two pig tibiae containing soft tissues. We compare low‐resolution peripheral quantitative computed tomography (pQCT) parameters and high‐resolution micro CT (μCT) in homologous single slices at 4% bone length and compare results to our novel Bone Ratio Predictor (BRP) method.ResultsRegression slopes between linear attenuation coefficients of low‐resolution pQCT images and bone area/total area (BA/TA) of high‐resolution μCT scans differ substantially between geographical subsamples, presumably due to diagenesis. BRP accurately predicts BA/TA (R2= .97) and eliminates the geographic clustering. BRP accurately estimates BA/TA in pigs containing soft tissues (R2= 0.98) without requiring knowledge of true density or phantom calibration of the scans.DiscussionBRP allows automated comparison of image data from different image modalities (pQCT, μCT) using different energy settings, in archeological bone and wet specimens. The method enables low‐resolution data generated in vivo to be compared with the fossil and archaeological record. Such experimental approaches would substantially improve behavioral inferences based on trabecular bone microstructure.