BOVINE IMMUNODEFICIENCY VIRUS - IMMUNOCHEMICAL CHARACTERIZATION AND SEROLOGICAL SURVEY
BOVINE IMMUNODEFICIENCY VIRUS - IMMUNOCHEMICAL CHARACTERIZATION AND SEROLOGICAL SURVEY
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DOI：
10.1099/0022-1317-72-12-2923
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发表时间：
1991-12-01
影响因子：
3.8
通讯作者：
KOOLEN, M
中科院分区：
文献类型：
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作者：
HORZINEK, M;KELDERMANS, L;KOOLEN, M
通过等密度离心纯化牛免疫缺陷病毒（BIV）;使用逆转录酶测定和p26特异性单克隆抗体ELISA在梯度级分中监测病毒活性。在符合的峰级分（密度约1.17 μ g/ml）中，使用实验性BIV感染后的小牛血清通过Western印迹检测到M（r）值为26 K、17 K、53 K、14 K和100 K（强度递减）的蛋白。当957随机收集的牛血清从荷兰进行了测试，间接免疫荧光和确认使用蛋白质印迹和/或放射免疫沉淀，1.4%出现血清阳性。因此，BIV感染在一个欧洲牛群中并不罕见。
Bovine immunodeficiency virus (BIV) was purified by isodensity centrifugation; viral activities were monitored in gradient fractions using the reverse transcriptase assay and a p26-specific monoclonal antibody ELISA. In the coincident peak fractions (density about 1.17 g/ml) proteins with M(r) values of 26K, 17K, 53K, 14K and 100K (with decreasing intensity) were detected by Western blotting using serum of a calf after experimental BIV infection. When 957 randomly collected cattle sera from The Netherlands were tested by indirect immunofluorescence and confirmed using Western blot and/or radioimmunoprecipitation, 1.4% appeared seropositive. Thus BIV infection is not uncommon in one European cattle population.