Lactogenic hormones increase epidermal growth factor messenger RNA content of mouse mammary glands.
Lactogenic hormones increase epidermal growth factor messenger RNA content of mouse mammary glands.
复制标题
催乳激素增加小鼠乳腺表皮生长因子信使 RNA 的含量。
DOI：
10.1016/0006-291x(91)92045-l
复制
发表时间：
1991
影响因子：
3.1
通讯作者：
Sheffield,LG
中科院分区：
文献类型：
--
作者：
Fenton,SE;Sheffield,LG
表皮生长因子（EGF）是已知刺激乳腺上皮细胞增殖，已确定在牛奶和哺乳期乳腺上皮细胞中表达。本研究探讨激素控制的EGF mRNA在小鼠乳腺。Prepro-EGF mRNA（4.7 kb）在哺乳期检测（并在此期间显着增加），而较小的EGF样RNA（0.5 kb）是在处女和怀孕小鼠的乳腺中的最高水平。4.7 kb RNA被聚腺苷酸化，而0.5 kb RNA没有。在类固醇致敏小鼠的乳腺器官培养物中，胰岛素+氢化可的松和胰岛素+催乳素+氢化可的松的组合增加了prepro-EGF和β-酪蛋白mRNA的表达。当氢化可的松存在时，乳腺EGF样RNA（0.5 kb条带）含量减少。我们的结论是prepro-EGF mRNA在小鼠乳腺组织中的表达是催乳激素和氢化可的松的控制下。
Epidermal growth factor (EGF) is known to stimulate mammary epithelial proliferation, has been identified in milk and is expressed in lactating mammary epithelia. This study examined hormonal control of EGF mRNA in mammary glands of mice. Prepro-EGF mRNA (4.7 kb) was detected during lactation (and increased significantly during this period), whereas a smaller EGF-like RNA (.5 kb) was at highest levels in mammary glands of virgin and pregnant mice. The 4.7 kb RNA was polyadenylated, whereas .5 kb RNA was not. In mammary gland organ cultures from steroid-primed mice, the combinations of insulin+hydrocortisone and insulin+prolactin+hydrocortisone increased both prepro-EGF and β-casein mRNA expression. When hydrocortisone was present there was a decrease in mammary gland content of EGF-like RNA (.5 kb band). We conclude that prepro-EGF mRNA expression in mouse mammary tissue is under the control of the lactogenic hormones prolactin and hydrocortisone.