Physiological and biochemical changes in naturally and artificially aged cotton seeds
Physiological and biochemical changes in naturally and artificially aged cotton seeds
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DOI：
10.15258/sst.2006.34.2.01
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发表时间：
2006-01-01
影响因子：
1.4
通讯作者：
Jose, I. C.
中科院分区：
文献类型：
--
作者：
Freitas, R. A.;Dias, D. C. F. S.;Jose, I. C.
研究了棉籽在自然老化和人工老化过程中的生理生化变化。棉花种子IAC-20 RR在环境条件下和冷藏室（10 +/- 2 ℃）中储存12个月。通过将种子在42 ℃和100%RH的培养箱中储存0、24、48、72、96和120小时来诱导人工老化。种子的生理质量（发芽，加速老化和冷发芽试验）和生化变化（脂肪氧合酶和酸性磷酸酶活性和脂质含量）后，每个人工老化期和两个月的间隔，为存储的种子。随着人工老化和自然老化的加剧，棉种的生活力、活力、脂氧合酶和酸性磷酸酶活性以及脂类含量均下降，尤其是在环境条件下贮藏的棉种。冷发芽试验、脂肪含量、酸性磷酸酶和脂肪氧合酶活性是检测自然老化或人工老化种子劣变的较好指标。
This study was done to determine the physiological and biochemical changes occurring in cotton seed during natural and artificial aging. Cotton seeds cv. IAC-20 RR were stored for 12 months under environmental condition and in a cold room (10 +/- 2 degrees C). The artificial aging was induced by storing seeds for 0, 24, 48, 72, 96 and 120 hours, in an incubator at 42 degrees C and 100% RH. Physiological quality of seeds (germination, accelerated aging and cool germination tests) and biochemical changes (lipoxygenase and acid phosphatase activity and lipid content) were determined after each artificial aging period and at two month interval for stored seeds. The viability, vigour, lipoxygenase and acid phosphatase activity and lipid content of cotton seeds decreased with the increasing artificial or natural aging, especially in seeds stored under environmental condition. Cool germination test, lipid content, acid phosphatase and lipoxygenase activities were good parameters for detecting deterioration of naturally or artificially aged seed lots.