CHARACTERIZATION OF PHOSPHATASE IN MARIGOLD ROOTS INFECTED WITH VESICULAR-ARBUSCULAR MYCORRHIZAL FUNGI
CHARACTERIZATION OF PHOSPHATASE IN MARIGOLD ROOTS INFECTED WITH VESICULAR-ARBUSCULAR MYCORRHIZAL FUNGI
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DOI：
10.1080/00380768.1994.10413299
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发表时间：
1994-06-01
影响因子：
2
通讯作者：
YOSHIDA, T
中科院分区：
文献类型：
--
作者：
EZAWA, T;YOSHIDA, T
研究了万寿菊（TagetespatulaL.）根的关系进行了调查菌根生长促进宿主植物。在生长室中培养了具有菌根和非菌根的万寿菊植株，从2 ~ 10周龄的植株根部提取酶液，通过凝胶电泳技术从侵染开始（4周龄植株）的菌根根提取液中检测到侵染特异性磷酸酶（ISP 10）。以4 ~ 8周龄的番茄为试材，研究了不同磷浓度的2种液肥对番茄内生真菌活性的影响及其对番茄菌根生长的促进作用。不施磷肥的植株比施磷肥的植株表现出更高的菌根植株地上部鲜重（SFW）和更高的ISTRO活性。ISO14000活动的综合价值通过硫酸铵、疏水层析和凝胶过滤部分纯化了ISTRO，其特征如下：最适pH为7.5，1 mM磷酸根离子抑制一半活性，该酶能有效地水解肌苷5 '-二磷酸，对多聚磷酸有一定的水解作用，并讨论了该酶作为菌根植物生长反应指示剂的可能性及其代谢作用。
The phosphatase specific to the infection of vesicular-arbuscular mycorrhizal fungi (Glomus etunicatum Becker & Gerdemann) in marigold (Tagetes patula L.) roots was investigated in relation to the mycorrhizal growth promotion in host plant. Mycorrhizal and non-mycorrhizal marigold plants were cultured in a growth chamber, and the enzyme solution was extracted from 2- to 10-week-old plant roots.The infection-specific phosphatase (ISPase) was detected in the mycorrhizal root extract from the beginning of the infection (4-week-old plant) by an electrophoretical technique. The activity increased as the infection rate increased, and decreased at the stationary phase of the host growth when the infection rate was still high.The effect of phosphorus (P) fertilization on the ISPase activity and the mycorrhizal growth promotion in host was investigated in 4- to 8-week-old plants using two kinds of liquid fertilizers with different P concentrations. The plants without P-fertilization showed more increase in the shoot fresh weight (SFW) of mycorrhizal plant and higher ISPase activity than the plant with P supply. The integrated values of the ISPase activity (from 0 to given week) were highly correlated with the increase in the SFW of the mycorrhizal plant.The ISPase was partially purified by ammonium sulfate, hydrophobic chromatography, and gel filtration, and characterized as follows: the optimum pH was 7.5, 1 mM phosphate ion inhibited half of the activity, the enzyme hydrolyzed inosine 5'-diphosphate effectively and polyphosphate moderately.The possibility of using ISPase as an indicator for the growth response of mycorrhizal plants and the metabolic role of this enzyme were discussed.