REGULATION OF PHAGOCYTOSIS AND [CA2+]I FLUX BY DISTINCT REGIONS OF AN FC RECEPTOR
REGULATION OF PHAGOCYTOSIS AND [CA2+]I FLUX BY DISTINCT REGIONS OF AN FC RECEPTOR
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DOI：
10.1126/science.1837175
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发表时间：
1991-12-20
期刊：
影响因子：
56.9
通讯作者：
UNKELESS, JC
中科院分区：
文献类型：
--
作者：
ODIN, JA;EDBERG, JC;UNKELESS, JC
多价免疫球蛋白 G 复合物与巨噬细胞上的 Fc 受体 (Fc-gamma-R) 结合可激活多种免疫功能。小鼠巨噬细胞，而非成纤维细胞系，转染人 Fc-γ-RIIA 介导的吞噬作用，并在人 Fc-γ-RIIA 交联后产生细胞内 Ca2+ 浓度 ([Ca2+]i) 流动。表达缺乏 17 个羧基末端氨基酸的截短受体的转染巨噬细胞吞噬小抗体复合物。然而，只有野生型转染子吞噬标记的红细胞并流动[Ca2+]i。因此，人Fc-γ-RIIA的胞质结构域包含不同的功能区域。
The binding of multivalent immunoglobulin G complexes to Fc receptors (Fc-gamma-Rs) on macrophages activates multiple immune functions. A murine macrophage cell fine, but not a fibroblast cell line, that was transfected with human Fc-gamma-RIIA mediated phagocytosis and an intracellular Ca2+ concentration ([Ca2+]i) flux upon cross-linking of human Fc-gamma-RIIA. Transfected macrophages that expressed a truncated receptor lacking 17 carboxy-terminal amino acids phagocytosed small antibody complexes. However, only wild-type transfectants phagocytosed labeled erythrocytes and fluxed [Ca2+]i. Thus, the cytoplasmic domain of human Fc-gamma-RIIA contains distinct functional regions.