Contrasting phylogeographic histories between the continent and islands of East Asia: Massive mitochondrial introgression and long-term isolation of hares (Lagomorpha: Lepus)
Contrasting phylogeographic histories between the continent and islands of East Asia: Massive mitochondrial introgression and long-term isolation of hares (Lagomorpha: Lepus)
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DOI：
10.1016/j.ympev.2019.04.003
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发表时间：
2019-07-01
影响因子：
4.1
通讯作者：
Suzuki, Hitoshi
中科院分区：
文献类型：
--
作者：
Kinoshita, Gohta;Nunome, Mitsuo;Suzuki, Hitoshi
兔属的野兔分布于世界各地，物种间的渐渗杂交被认为是普遍的，导致网状进化和分类混乱。在这里，我们对东亚的以下野兔物种进行了地理分布分析：L。提米迪斯湖mandshuricus，L. coreanus和L.从俄罗斯远东、韩国和日本采集的短尾龙。分析了1个线粒体DNA和8个核基因位点的核苷酸序列，并从数据库中添加了中国野兔的序列。所有的核DNA分析都支持三个类群的明确分离：L.提米迪斯湖brachyurus和L.含L.朝鲜另一方面，两个L. timidus谱系为L. mandshuricus复合体。L.的北方种群。mandshuricus复合体主要与L. timidus，可能是末次冰期以来，而南方的L. mandshuricus复合体经历了从另一个L. timidus谱系与北海道种群有关，可能在末次冰期之前。与大陆野兔相反，在L.在日本群岛的brachyurus，这表明东亚野兔血统之间最古老的分歧。我们的研究结果表明，在环日本海地区的冰川间冰期气候变化促进分布的变化和渐渗杂交大陆野兔物种，而该地区的地理结构有助于长期隔离野兔的岛屿上，防止物种间的基因流。
Hares of the genus Lepus are distributed worldwide, and introgressive hybridization is thought to be pervasive among species, leading to reticulate evolution and taxonomic confusion. Here, we performed phylogeographic analyses of the following species of hare across East Asia: L. timidus, L. mandshuricus, L. coreanus, and L. brachyurus collected from far-eastern Russia, South Korea, and Japan. Nucleotide sequences of one mitochondrial DNA and eight nuclear gene loci were examined, adding sequences of hares in China from databases. All nuclear DNA analyses supported the clear separation of three phylogroups: L. timidus, L. brachyurus, and the L. mandshuricus complex containing L. coreanus. On the other hand, massive mitochondrial introgression from two L. timidus lineages to the L. mandshuricus complex was suggested in continental East Asia. The northern population of the L. mandshuricus complex was mainly associated with introgression from the continental lineage of L. timidus, possibly since the last glacial period, whereas the southern population of the L. mandshuricus complex experienced introgression from another L. timidus lineage related to the Hokkaido population, possibly before the last glacial period. In contrast to continental hares, no evidence of introgression was found in L. brachyurus in the Japanese Archipelago, which showed the oldest divergence amongst East Asian hare lineages. Our findings suggest that glacial-interglacial climate changes in the circum-Japan Sea region promoted distribution shifts and introgressive hybridization among continental hare species, while the geographic structure of the region contributed to long-term isolation of hares on the islands, preventing inter-species gene flow.