PtdIns 3-Kinase Orchestrates Autophagosome Formation in Yeast.
PtdIns 3-Kinase Orchestrates Autophagosome Formation in Yeast.
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DOI：
10.1155/2011/498768
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发表时间：
2011
影响因子：
5.3
通讯作者：
Ohsumi Y
中科院分区：
文献类型：
--
作者：
Obara K;Ohsumi Y
真核细胞可以大量地将它们自己的细胞质内容物运输到一个裂解室，即液泡/溶酶体，以便通过一个称为自噬的保守系统进行循环。自噬的关键过程是将细胞质内容物隔离在双层膜结构中，即自噬小体。自噬小体的形成需要ATG(自噬相关)蛋白质和脂类分子的精心合作。磷脂酰肌醇(PtdIns)被磷脂酰肌醇(PtdIns)3-激酶Vps34磷酸化是协调ATG蛋白和脂质分子的关键步骤。Vps34形成两种不同的蛋白质复合体，其中只有一种参与了自噬膜的形成。在自噬的诱导下，PtdIns(3)P的酶产物PtdIns(3)P通过自噬大量地运输到液泡的管腔中。PtdIns(3)P富含在自噬小体的内膜上。PtdIns(3)P招募atg18−atg2复合体和可能的其他atg蛋白到自噬膜上，从而协调脂分子和atg蛋白。
Eukaryotic cells can massively transport their own cytoplasmic contents into a lytic compartment, the vacuole/lysosome, for recycling through a conserved system called autophagy. The key process in autophagy is the sequestration of cytoplasmic contents within a double-membrane structure, the autophagosome. Autophagosome formation requires the elaborate cooperation of Atg (autophagy-related) proteins and lipid molecules. Phosphorylation of phosphatidylinositol (PtdIns) by a PtdIns 3-kinase, Vps34, is a key step in coordinating Atg proteins and lipid molecules. Vps34 forms two distinct protein complexes, only one of which is involved in generating autophagic membranes. Upon induction of autophagy, PtdIns(3)P, the enzymatic product of PtdIns 3-kinase, is massively transported into the lumen of the vacuole via autophagy. PtdIns(3)P is enriched on the inner membrane of the autophagosome. PtdIns(3)P recruits the Atg18−Atg2 complex and presumably other Atg proteins to autophagic membranes, thereby coordinating lipid molecules and Atg proteins.