Osteogenic response of human MSCs and osteoblasts to hydrophilic and hydrophobic nanostructured titanium implant surfaces
Osteogenic response of human MSCs and osteoblasts to hydrophilic and hydrophobic nanostructured titanium implant surfaces
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DOI：
10.1002/jbm.a.35852
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发表时间：
2016-12-01
影响因子：
4.9
通讯作者：
Schwartz, Zvi
中科院分区：
文献类型：
--
作者：
Lotz, Ethan M.;Olivares-Navarrete, Rene;Schwartz, Zvi
通过喷砂和酸蚀钛（Ti）形成的微结构种植体表面支持骨整合。通过在水溶液中储存以保持亲水性，这种效果进一步增强，但这也会导致表面纳米结构的形成。本研究的目的是评估纳米结构对改善成骨细胞谱系对亲水微结构钛的成骨反应的贡献。将人间充质干细胞（MSCs）和正常人成骨细胞（NHOsts）分别培养在非纳米结构/疏水（SLA）、纳米结构/亲水性（modSLA）或纳米结构/疏水（SLAnano）钛表面。与modSLA相比，XPS显示SLA和SLAnano上的碳含量升高。接触角测量表明，只有modSLA是亲水的。共聚焦激光显微镜显示平均表面粗糙度的微小差异。SEM显示在modSLA和SLAnano上存在纳米结构。MSCs和NHOst细胞在基质上表现出相似的形态，在modSLA上成骨细胞分化和成熟程度最高。这些结果表明，当适当的微观结构存在时，亲水性可能比在水环境中储存过程中产生的纳米结构在刺激MSC和NHOst成骨细胞分化和成熟方面发挥更大的作用。(c) 2016 Wiley Periodicals, Inc.；[J]中国生物医学工程学报，2016,31(4):387 - 398。
Microstructured implant surfaces created by grit blasting and acid etching titanium (Ti) support osseointegration. This effect is further enhanced by storing in aqueous solution to retain hydrophilicity, but this also leads to surface nanostructure formation. The purpose of this study was to assess the contributions of nanostructures on the improved osteogenic response of osteoblast lineage cells to hydrophilic microstructured Ti. Human mesenchymal stem cells (MSCs) and normal human osteoblasts (NHOsts) were cultured separately on non-nanostructured/hydrophobic (SLA), nanostructured/hydrophilic (modSLA), or nanostructured/hydrophobic (SLAnano) Ti surfaces. XPS showed elevated carbon levels on SLA and SLAnano compared to modSLA. Contact angle measurements indicated only modSLA was hydrophilic. Confocal laser microscopy revealed minor differences in mean surface roughness. SEM showed the presence of nanostructures on modSLA and SLAnano. MSCs and NHOst cells exhibited similar morphology on the substrates and osteoblastic differentiation and maturation were greatest on modSLA. These results suggest that when the appropriate microstructure is present, hydrophilicity may play a greater role in stimulating MSC and NHOst osteoblastic differentiation and maturation than the presence of nanostructures generated during storage in an aqueous environment. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 3137-3148, 2016.