EFFECTS OF THE LENS CAPSULE ON CELLULAR FLATTENING, CELL-GROWTH AND EXPRESSION OF THE DIFFERENTIATED TRAITS OF CHONDROCYTES CULTURED INVITRO
EFFECTS OF THE LENS CAPSULE ON CELLULAR FLATTENING, CELL-GROWTH AND EXPRESSION OF THE DIFFERENTIATED TRAITS OF CHONDROCYTES CULTURED INVITRO
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DOI：
10.1111/j.1440-169x.1979.00437.x
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发表时间：
1979-01-01
影响因子：
2.5
通讯作者：
TSUNEMATSU, Y
中科院分区：
文献类型：
--
作者：
TSUNEMATSU, Y
研究了鸡胚软骨细胞在鸡胚透镜囊上培养的形态、生长和分化。当在含有胎血清的MEM（最低必需培养基）中孵育时，透镜囊表面的软骨细胞变平，伪足延长，但大多数细胞在塑料培养皿表面保持球形。透镜囊促进软骨细胞扁平化。当在Hams F12培养基中生长时，透镜囊上的细胞变平程度更大，并且细胞发展成纤维细胞的特征，而没有任何可检测到的软骨细胞的特征，尽管它们的生长速率没有增强。当细胞从透镜囊分离并在塑料基质上生长时，透镜囊对该培养基中分化的这种抑制作用被逆转。
The morphology, growth and differentiation of [chick embryo] chondrocytes cultured on the chick embryo lens capsule were studied. When incubated in Eagle''s MEM [minimum essential medium] with fetal serum, chondrocytes on the surface of the lens capsule became flattened with extended pseudopodia, but most cells remained spherical on the surface of a plastic dish. The lens capsule promoted cellular flattening of chondrocytes. When grown in Ham''s F12 medium, flattening of cells on the lens capsule was greater and the cells developed the features of fibroblasts without any detectable characteristics of chondrocytes, although their growth rate was not enhanced. This inhibitory effect of the lens capsule on differentiation in this medium was reversed when the cells were separated from the lens capsule and grown on a plastic substrate.