Structure of a copper-mediated base pair in DNA
Structure of a copper-mediated base pair in DNA
复制标题
DOI：
10.1021/ja011822e
复制
发表时间：
2001-12-12
影响因子：
15
通讯作者：
Schultz, PG
中科院分区：
文献类型：
--
作者：
Atwell, S;Meggers, E;Schultz, PG
最近用含有互补吡啶-2，6-二羧酸（Dipic）和吡啶（Py）碱基的核苷酸证明了通过金属配位的稳定和选择性DNA碱基配对（Meggers，E.;荷兰，P.L. Tolman; W. B. Romesberg，F.E.; P.G.舒尔茨J. Am. 2000，122，10714 - 10715）。为了理解将这种新的碱基对引入DNA的结构后果，我们已经解决了含有金属碱基对的双链体的晶体结构，该结构显示碱基如设计的那样配对，但处于Z-DNA构象。该结构还提供了一个结构解释的B-到Z-DNA的转换在这个双链体，进一步的解决方案的研究表明，金属碱基对是兼容的Z-或B-DNA构象，这取决于双链体序列。
Stable and selective DNA base pairing by metal coordination was recently demonstrated with nucleotides containing complementary pyridine-2,6-dicarboxylate (Dipic) and pyridine (Py) bases (Meggers, E.; Holland, P.L. Tolman; W.B. Romesberg, F.E.; Schultz, P.G. J. Am. Chem. Soc. 2000, 122, 10714-10715). To understand the structural consequences of introducing this novel base pair into DNA we have solved the crystal structure of a duplex containing the metallo-base pair, The structure shows that the bases pair as designed, but in a Z-DNA conformation. The structure also provides a structural explanation for the B- to Z-DNA transition in this duplex, Further solution studies demonstrate that the metallo-base pair is compatible with Z- or B-DNA conformations, depending on the duplex sequence.