Selective in vivo depletion of CD4(+) T lymphocytes with anti-CD4 monoclonal antibody during acute infection of calves with Anaplasma marginale.
Selective in vivo depletion of CD4(+) T lymphocytes with anti-CD4 monoclonal antibody during acute infection of calves with Anaplasma marginale.
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在小牛急性感染边缘无形体期间，用抗 CD4 单克隆抗体选择性体内清除 CD4( ) T 淋巴细胞。
DOI：
10.1128/cdli.9.2.417-424.2002
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发表时间：
2002
期刊：
影响因子：
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通讯作者：
Knowles,DonaldP
中科院分区：
文献类型：
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作者：
Valdez,ReginaldA;McGuire,TravisC;Brown,WendyC;Davis,WilliamC;Jordan,JeffreyM;Knowles,DonaldP
为了研究 CD4+T 淋巴细胞在急性无形体病期间的体内作用，通过抗 CD4 单克隆抗体 (MAb) 处理，选择性去除胸腺切除的小牛中的 CD4+T 淋巴细胞，然后在两个连续实验（实验 1 和 2）中用边缘无形体佛罗里达株感染。胸腺切除的小牛在第一周内接受总共 5.0 mg 抗 CD4 MAb/kg 体重的治疗，随后每周两次以 0.3 mg/kg 的剂量进行治疗，持续 7 周，导致在 8 周的研究期间，血液、脾脏和外周淋巴结中的 CD3+CD4+ 和 CD4+CD45R+（初始）T 淋巴细胞显着减少（与对照组的结果相比）。用亚类匹配的单克隆抗体治疗胸腺切除的对照小牛。所有小牛在实验性感染 A 后都出现寄生虫病和发热。边缘，细胞堆积体积（PCV）的减少与寄生虫血症的峰值一致。治疗组之间未观察到 PCV 或寄生虫血症的显着差异。用抗 CD4 MAb 治疗的胸腺切除小牛能够产生抗 A。边际抗体反应，尽管在实验 2 中，抗 CD4 MAb 处理的小牛的免疫球蛋白 G1 (IgG1) 降低了四到六倍，并且没有检测到 IgG2 抗 A。与用亚类匹配的 MAb 处理的胸腺切除对照小牛相比，边缘主要表面蛋白 2 特异性抗体滴度。在达到 CD4+-T 淋巴细胞消除和实验性无形体病诱导的水平时，切除胸腺的抗 CD4 MAb 处理的小牛能够控制急性无形体病。这与 CD4+T 淋巴细胞显着减少将消除对急性感染的抵抗力的预测相反。
To investigate the in vivo role of CD4+T lymphocytes during acute anaplasmosis, thymectomized calves were selectively depleted of CD4+T lymphocytes by treatment with anti-CD4 monoclonal antibody (MAb) and were then infected with the Florida strain ofAnaplasma marginalein two sequential experiments (experiments 1 and 2). Treatment of thymectomized calves with a total of 5.0 mg of anti-CD4 MAb/kg of body weight during the 1st week followed by 0.3-mg/kg doses administered twice weekly for 7 weeks resulted in significant depletion of CD3+CD4+and CD4+CD45R+(naive) T lymphocytes from blood, spleen, and peripheral lymph nodes for the duration of the 8-week study, compared to the results for thymectomized control calves treated with a subclass-matched MAb. All calves became parasitemic and pyretic following experimental infection withA. marginale, and decreases in packed cell volume (PCV) coincided with peak parasitemia. No significant differences in PCV or parasitemia were observed between treatment groups. Thymectomized calves treated with anti-CD4 MAb were able to mount an anti-A. marginaleantibody response, although in experiment 2, anti-CD4 MAb-treated calves had four- to sixfold lower immunoglobulin G1 (IgG1) and no detectable IgG2 anti-A. marginalemajor surface protein 2-specific antibody titers compared to thymectomized control calves treated with a subclass-matched MAb. At the level of CD4+-T-lymphocyte depletion achieved and experimental anaplasmosis induced, thymectomized anti-CD4 MAb-treated calves were able to control acute anaplasmosis. This was in contrast to the prediction that significant depletion of CD4+T lymphocytes would abrogate resistance to acute infection.