Transcriptome analysis of rice leaves in response toRhizoctonia solaniinfection and reveals a novel regulatory mechanism
Transcriptome analysis of rice leaves in response toRhizoctonia solaniinfection and reveals a novel regulatory mechanism
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DOI：
10.1007/s11816-020-00630-9
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发表时间：
2020-06-09
影响因子：
2.4
通讯作者：
Xuan, Yuan Hu
中科院分区：
文献类型：
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作者：
Yuan, De Peng;Xu, Xiao Feng;Xuan, Yuan Hu
水稻纹枯病严重影响水稻生产，但其防御机制尚不清楚。为了解水稻对ShB的防御机制，以接种水稻立枯病菌（Rhizoctonia solani）48 h后的叶片为材料，进行了RNA测序分析。其中3417个基因表达上调，2532个基因表达下调（> 2倍或< 1/2）。此外，差异表达的基因通过基因本体论（GO），京都基因和基因组百科全书（KEGG）和MapMan分析进行分类。59个GO术语和7个KEGG通路显著富集。一个MapMan分析表明，植物激素和代谢途径显着改变。有趣的是，包括WRKY、MYB和NAC家族成员在内的359个转录因子以及包括ABC、MFS和SWEET在内的239个转运蛋白基因的表达水平在对R的反应中发生了显著变化。SolaniAG 1-IA接种。此外，OsWRKY 53和OsAKT 1负调控水稻对水稻抗病性的防御反应。分别对OsWRKY 53进行了功能获得研究和对OsAKT 1进行了功能丧失研究。此外，一些差异表达的基因含有R.在它们的启动子区域中的茄属植物响应性表达调节元件。综上所述，我们的分析提供了有价值的信息，进一步的防御机制对ShB的研究，在这项研究中确定的候选基因将是有用的资源，为未来的育种，以提高对ShB的抗性。
Sheath blight disease (ShB) severely affects rice production; however, the details of defense against ShB remain unclear. To understand the rice defense mechanism against ShB, an RNA sequencing analysis was performed usingRhizoctonia solaniinoculated rice leaves after 48 h of inoculation. Among them, 3417 genes were upregulated and 2532 were downregulated when compared with the control group (> twofold or < 1/2). In addition, the differentially expressed genes were classified via Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and MapMan analyses. Fifty-nine GO terms and seven KEGG pathways were significantly enriched. A MapMan analysis demonstrated that the phytohormone and metabolic pathways were significantly altered. Interestingly, the expression levels of 359 transcription factors, including WRKY, MYB, and NAC family members, as well as 239 transporter genes, including ABC, MFS, and SWEET, were significantly changed in response toR. solaniAG1-IA inoculation. Additionally, OsWRKY53 and OsAKT1 negatively regulate the defense response in rice againstR. solanivia gain of function study forOsWRKY53and loss of function study forOsAKT1, respectively. Furthermore, several differentially expressed genes containR. solani-responsivecisacting regulatory elements in their promoter regions. Taken together, our analyses provide valuable information for the additional study of the defense mechanisms against ShB, and the candidate genes identified in this study will be useful resource for future breeding to enhance resistance against ShB.