Quantification of effects of global ischemia on dynamics of ventricular fibrillation in isolated rabbit heart
Quantification of effects of global ischemia on dynamics of ventricular fibrillation in isolated rabbit heart
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DOI：
10.1161/01.cir.98.16.1688
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发表时间：
1998-10-20
期刊：
影响因子：
37.8
通讯作者：
Jalife, J
中科院分区：
文献类型：
--
作者：
Mandapati, R;Asano, Y;Jalife, J
背景-心室颤动（VE）导致心脏全脑缺血。在发作1至2分钟后，VF率降低，并且看起来更有组织。本研究的目的是确定无血流全脑缺血对非线性波动力学的影响，并建立缺血诱导的VF率减慢的机制。方法和结果-使用2种方案研究了Langendorff灌注兔心脏中VF的激活模式：（1）全脑缺血15分钟后再灌注（n=7）;（2）用5 μ mol/L河豚毒素（TTX）灌流后洗脱（n=3），降低兴奋性。视频成像（约7500像素每帧;每秒240帧）与电压敏感染料，EGG，和信号处理（快速傅立叶变换）用于分析。VF的主频从对照组的13.5 ± 1.3 Hz下降到全脑缺血5分钟时的9.3 ± 1.4 Hz（P
Background-Ventricular fibrillation (VE) leads to global ischemia of the heart. After 1 to 2 minutes of onset, the VF rate decreases and appears more organized. The objectives of this study were to determine the effects of no-flow global ischemia on nonlinear wave dynamics and establish the mechanism of ischemia-induced slowing of the VF rate.Methods and Results-Activation patterns of VF in the Langendorff-perfused rabbit heart were studied with the use of 2 protocols: (1) 15 minutes of no-flow global ischemia followed by reperfusion (n=7) and (2) decreased excitability induced by perfusion with 5 mu mol/L of tetrodotoxin (TTX) followed by washout (n=3). Video imaging (approximate to 7500 pixels per frame; 240 frames per second) with a voltage-sensitive dye, EGG, and signal processing (fast Fourier transform) were used for analysis. The dominant frequency of VF decreased from 13.5+/-1.3 during control to 9.3+/-1.4 Hz at 5 minutes of global ischemia (P