Initial experience of correlating diffusion spectral parameters with histopathologic indexes in murine colorectal tumor homografts.
Initial experience of correlating diffusion spectral parameters with histopathologic indexes in murine colorectal tumor homografts.
复制标题
将小鼠结直肠肿瘤同种移植物的扩散光谱参数与组织病理学指标相关联的初步经验
DOI：
10.2147/ott.s127283
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发表时间：
2017
影响因子：
4
通讯作者：
Sun YS
中科院分区：
文献类型：
--
作者：
Zhang XY;Li XT;Sun J;Sun YS
目的探讨连续分布弥散加权图像（DWI）衍生参数与组织病理指标的相关性。方法选取54只HCT-116结直肠肿瘤小鼠进行分析；连续观察12只，断点观察42只。所有小鼠随机分为放疗组和非放疗组。根据放疗方案在不同时间点（基线、24小时、48小时、72小时、7天、14天、28天）进行光学成像和MRI检查。对连续观测数据进行分析，显示放疗组与非放疗组光学参数和mr - dwi衍生参数（光子数、D_max、全宽半最大值[FWHM]和表观扩散系数[ADC]值）动态变化趋势的差异。断点观察数据用于分析组织病理学指标与dwi衍生参数之间的相关性。结果放疗组与非放疗组的光子数、D_max、FWHM、ADC值的变化趋势有显著差异，尤其是在早期时间点。Ki67与D_max、FWHM、ADC值百分比变化呈中度负相关（相关系数分别为0.632、0.449、0.586，P<0.001、P=0.008、P<0.001）。survivin与D_max和ADC值变化百分比呈中度负相关（相关系数分别为0.496和0.473，P=0.004和P=0.006）。结论连续分布的dwi参数能在一定程度上反映肿瘤的组织学行为，是早期无创监测肿瘤细胞凋亡和增殖的潜在指标。
Purpose To determine the correlation between continuously distributed diffusion-weighted image (DWI)-derived parameters and histopathologic indexes. Methods Fifty-four mice bearing HCT-116 colorectal tumors were included for analysis; 12 mice were used for continuous observation, and the other 42 mice were used for break-point observation. All mice were randomly divided into radiotherapy and non-radiotherapy groups. Optical imaging and MRI were performed at different time points according to radiotherapy regimen (baseline, 24 h, 48 h, 72 h, 7 d, 14 d, and 28 d). Continuous observation data were analyzed to show the difference of dynamic changing trends of optical and MR-DWI–derived parameters between radiotherapy and non-radiotherapy groups (photon numbers, D_max, full width half maximum [FWHM], and apparent diffusion coefficient [ADC] value). Break-point observation data were used to analyze the correlation between histopathologic indices and DWI-derived parameters. Results There was a significant difference in the changing trends of photon numbers, D_max, FWHM, and ADC value between radiotherapy and non-radiotherapy groups, especially at early time points. There was moderate negative correlation between Ki67 and percentage changes of D_max, FWHM, and ADC values (the correlation coefficients were 0.632, 0.449, and 0.586, P<0.001, P=0.008, and P<0.001, respectively). There was moderate negative correlation between survivin and percentage changes of D_max and ADC values (correlation coefficients were 0.496 and 0.473, P=0.004 and P=0.006, respectively). Conclusion The continuously distributed DWI-derived parameters could reflect histological behavior to some extent and, thus, are potential markers for early noninvasive monitoring of tumor cell apoptosis and proliferation.