Genetic diversity of the Mycobacterium tuberculosis Beijing family based on multiple genotyping profiles
Genetic diversity of the Mycobacterium tuberculosis Beijing family based on multiple genotyping profiles
复制标题
基于多重基因分型谱的结核分枝杆菌北京家族的遗传多样性。
DOI：
10.1017/s095026881500312x
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发表时间：
2016-06-01
影响因子：
4.2
通讯作者：
Wang, W.
中科院分区：
文献类型：
--
作者：
Liu, Y.;Wang, S.;Wang, W.
全球最流行的结核分枝杆菌(Mtb)菌株之一是北京型，它导致了结核病(TB)的大规模爆发。促进北京家庭毒株传播的特征尚不清楚，但据推测它们是通过谱系的进化获得的。为了解北京结核分枝杆菌属结核分枝杆菌的遗传多样性，探讨分枝杆菌散布重复单位-可变数目串联重复序列基因座在东亚多个地区的鉴别能力，对2009年6月1日至2010年11月31日在中国阜宁县登记的163株北京结核分枝杆菌进行了横断面研究。对分离的菌株进行15-Miru-VNTR基因座分型，并与已发表的北京分离株的Miru-VNTR图谱进行比较。对10个染色体位置的同义单核苷酸多态进行了分析。SNP和Miru-VNTR分型相结合可用于北京地区Mtb基因分型。上海的现代亚科与来自其他国家的毒株重叠，而古老的亚科在几个国家之间存在遗传差异。现代亚家族，尤其是ST10亚科占优势。Qub11b和其他4个基因座(Miru 26、Mtub21、Qub26、Mtub04)可用于区分北京地区的菌株。
Among the most prevalent Mycobacterium tuberculosis (Mtb) strains worldwide is the Beijing genotype, which has caused large outbreaks of tuberculosis (TB). Characteristics facilitating the dissemination of Beijing family strains remain unknown, but they are presumed to have been acquired through evolution of the lineage. To explore the genetic diversity of the Beijing family Mtb and explore the discriminatory ability of mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU-VNTR) loci in several regions of East Asia, a cross-sectional study was conducted with a total of 163 Beijing strains collected from registered TB patients between 1 June 2009 and 31 November 2010 in Funing County, China. The isolated strains were analysed by 15-MIRU-VNTR loci typing and compared with published MIRU-VNTR profiles of Beijing strains. Synonymous single nucleotide polymorphisms at 10 chromosomal positions were also analysed. The combination of SNP and MIRU-VNTR typing may be used to assess Mtb genotypes in areas dominated by Beijing strains. The modern subfamily in Shanghai overlapped with strains from other countries, whereas the ancient subfamily was genetically differentiated across several countries. Modern subfamilies, especially ST10, were prevalent. Qub11b and four other loci (MIRU 26, Mtub21, Qub26, Mtub04) could be used to discriminate Beijing strains.