Notch signaling directly controls cell proliferation in the Drosophila wing disc
Notch signaling directly controls cell proliferation in the Drosophila wing disc
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DOI：
10.1073/pnas.040576497
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发表时间：
2000-03-14
影响因子：
11.1
通讯作者：
Garcia-Bellido, A
中科院分区：
文献类型：
--
作者：
Baonza, A;Garcia-Bellido, A
Notch 信号传导参与形态发生过程中的细胞分化和模式形成。在果蝇翅膀中，Notch 活性调节背侧/腹侧边界的多个基因的表达，这被认为会引发翼细胞增殖。在这项工作中，我们展示了表达不同形式的 Notch 信号通路几个成员的细胞克隆的作用，这会导致 Notch 活性的改变。翼中Notch或其配体（Delta或Serrate）激活形式克隆的异位表达导致与异位翼缘出现相关的生长。这些产物由突变区域和周围的野生型细胞组成。然而，腹侧克隆中 Delta 的异位表达水平较低，会导致大量的生长，这些生长既与翼缘结构的生成无关，也与背/腹边界特征基因的表达无关。这些结果表明，Notch 活性直接参与细胞增殖，与其在背/腹边界形成中的作用无关。我们认为，非自主效应（在周围野生型细胞中诱导额外增殖和静脉分化）是由Notch异位表达引起的位置值模式调节造成的。
Notch signaling is involved in cell differentiation and patterning during morphogenesis, In the Drosophila wing, Notch activity regulates the expression of several genes at the dorsal/ventral boundary, and this is thought to elicit wing-cell proliferation. In this work, we show the eff ect of clones of cells expressing different forms of several members of the Notch signaling pathway, which result in an alteration of Notch activity. The ectopic expression in clones of activated forms of Notch or of its ligands (Delta or Serrate) in the wing causes outgrowths associated with the appearance of ectopic wing margins. These outgrowths consist of mutant territories and of surrounding wild-type cells. However, the ectopic expression of Delta, at low levels in ventral clones, causes large outgrowths that are associated neither with the generation of wing margin structures nor with the expression of genes characteristic of the dorsal/ventral boundary. These results suggest that Notch activity is directly involved in cell proliferation, independently of its role in the formation of the dorsal/ventral boundary. We propose that the nonautonomous effects (induction of extraproliferation and vein differentiation in the surrounding wild-type cells) result from pattern accommodation to positional values caused by the ectopic expression of Notch.