Activation of B1a Cells in Peritoneal Cavity by T Cell-Independent Antigen Expressed on Polymeric Micelle
Activation of B1a Cells in Peritoneal Cavity by T Cell-Independent Antigen Expressed on Polymeric Micelle
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聚合胶束上表达的 T 细胞依赖性抗原激活腹腔 B1a 细胞
DOI：
10.1002/jps.24397
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发表时间：
2015
影响因子：
3.8
通讯作者：
Manabu Sugai2 and Shunsaku Kimura1*
中科院分区：
文献类型：
--
作者：
Cheol Joo Kim1;Eri Hara2;Akira Shimizu2;Manabu Sugai2 and Shunsaku Kimura1*
由两亲性聚缩酚酸肽（poly（sarcosine）-block-poly（L-lactic acid））组成的聚合物胶束（Lactosome）被报道为T细胞非依赖性抗原。我们在这里表明，乳糖体反应B细胞主要发现在腹膜腔（PerC）。Lactosome免疫小鼠后，仅在脾脏和骨髓（BM）中发现抗体分泌细胞（ASC），而在PerC中未发现。酶联免疫斑点试验表明，脾脏中的优势ASCs为浆母细胞。5-溴-2 ′-脱氧尿苷（5-Bromo-2′-deoxyuridine，BrdU）检测显示，Lactosome刺激下，Lactosome反应性的B1 a细胞增殖，并大多数停留在Lactosome。这些数据表明，Lactosome与B细胞相互作用的主要位点在PerC中，并且一些活化的B细胞迁移到脾或BM中并在那里分化为浆母细胞。正如预期的那样，当从乳糖体免疫小鼠中收集PerC中的B1 a细胞并移植到重组激活基因2（RAG 2）-/-小鼠中时，在受体小鼠中观察到抗乳糖体免疫球蛋白M（IgM）的产生。因此认为Lactosome刺激的腹膜B1 a细胞是脾内持续浆母细胞的来源。
A polymeric micelle (Lactosome) composed of amphiphilic polydepsipeptide, poly(sarcosine)-block-poly(L-lactic acid), was reported as a T cell-independent antigen. We show here that Lactosome-responsive B cells are predominantly found in the peritoneal cavity (PerC). After immunization of mice with Lactosome, antibody-secreting cells (ASCs) are found only in spleen and bone marrow (BM), but not in PerC. The enzyme-linked immunospot assay shows that the dominant ASCs are plasmablasts in spleen. 5-Bromo-2′-deoxyuridine (BrdU) assay reveals that Lactosome-responsive peritoneal B1a cells proliferate by the stimulation of Lactosome and the majority of them stay there. These data indicate that the primary site for Lactosome to interact with B cells is in PerC, and some of activated B cells migrate into spleen or BM and differentiate into plasmablast there. As expected, when the B1a cells in PerC are collected from the Lactosome-immunized mice and are transplanted into recombination activating gene 2 (RAG2)-/-mice, the anti-Lactosome immunoglobulin M (IgM) production is observed in the recipient mice. It is therefore considered that the peritoneal B1a cells stimulated by Lactosome are the source of the sustained plasmablasts in spleen.