Microfluidic Photonic Integrated Circuits.
Microfluidic Photonic Integrated Circuits.
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DOI：
10.1117/12.807153
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发表时间：
2008-11-18
期刊：
影响因子：
--
通讯作者：
Lo YH
中科院分区：
文献类型：
--
作者：
Cho SH;Godin J;Chen CH;Tsai FS;Lo YH
我们报告了一种廉价的便携式芯片上实验室流式细胞仪系统的发展，其中微流体，光子学和声学集成在一起协同工作。该系统依赖于充满流体的二维片上光子元件，如透镜、孔径和平板波导，以实现照明激光束整形、光散射和荧光信号检测。散射光和荧光灯在被片上光学元件（如透镜和波导）收集和引导后由光电探测器检测到。检测到的光信号被实时输入和放大，并触发压电致动器，从而将目标样品定向到所需的储层中进行后续的高级分析。采用现场可编程门阵列（FPGA）实现实时闭环控制系统。该系统在芯片上实现高通量（1-10kHz操作）、高可靠性和低功耗（<1mW）荧光激活细胞分选（FACS）。这种微型制造的流式细胞仪在资源贫乏的环境中可以作为一种便携、廉价的即时护理设备。
We report on the development of an inexpensive, portable lab-on-a-chip flow cytometer system in which microfluidics, photonics, and acoustics are integrated together to work synergistically. The system relies on fluid-filled two-dimensional on-chip photonic components such as lenses, apertures, and slab waveguides to allow for illumination laser beam shaping, light scattering and fluorescence signal detection. Both scattered and fluorescent lights are detected by photodetectors after being collected and guided by the on-chip optics components (e.g. lenses and waveguides). The detected light signal is imported and amplified in real time and triggers the piezoelectric actuator so that the targeted samples are directed into desired reservoir for subsequent advanced analysis. The real-time, closed-loop control system is developed with field-programmable-gate-array (FPGA) implementation. The system enables high-throughput (1–10kHz operation), high reliability and low-powered (<1mW) fluorescence activated cell sorting (FACS) on a chip. The microfabricated flow cytometer can potentially be used as a portable, inexpensive point-of-care device in resource poor environments.