IMMUNOCHEMICAL ASSESSMENT OF MYCOTOXINS IN 1989 GRAIN FOODS - EVIDENCE FOR DEOXYNIVALENOL (VOMITOXIN) CONTAMINATION
IMMUNOCHEMICAL ASSESSMENT OF MYCOTOXINS IN 1989 GRAIN FOODS - EVIDENCE FOR DEOXYNIVALENOL (VOMITOXIN) CONTAMINATION
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DOI：
10.1128/aem.57.3.672-677.1991
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发表时间：
1991-03-01
影响因子：
4.4
通讯作者：
PESTKA, JJ
中科院分区：
文献类型：
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作者：
ABOUZIED, MM;AZCONA, JI;PESTKA, JJ
为了评估1988年美国干旱后人类食品供应中霉菌毒素污染的可能性，1989年夏天从零售商店购买了92份谷物食品样品，并采用基于单克隆抗体的竞争酶联免疫吸附试验（ELISA）对黄曲霉毒素B1、玉米赤霉烯酮和脱氧雪腐菌烯醇（DON [vomitoxin]）进行了调查。只有一个样品（荞麦粉）被发现含有黄曲霉毒素B1（12纳克/克），而26%的样品中发现了玉米赤霉烯酮，平均浓度为19纳克/克。相比之下，DON酶联免疫吸附试验在50%的样品中呈阳性，检测水平为1.0 μ g/g。63%至88%的玉米谷物、小麦粉/松饼混合物、大米谷物和玉米粉/松饼混合物的DON检测结果呈阳性，而25至50%的燕麦谷物、小麦和燕麦饼干/饼干、玉米片、爆米花和混合谷物的DON检测结果呈阳性。阳性样品的DON平均含量为4.0 mu-g/g，最低和最高含量分别为1.2和19 mu-g/g。当ELISA阳性样品也用高效液相色谱分析时，发现两种方法之间有很强的相关性。用气相色谱-质谱联用法定量证实了玉米粉和杂粮中DON的含量最高，分别为19 μ g/g和16 μ g/g。结果表明，1989年零售食品中DON的浓度超过了以前市场调查中发现的浓度，并且在实验中与动物健康受损有关。
To assess the potential for mycotoxin contamination of the human food supply following the 1988 U.S. drought, 92 grain food samples were purchased from retail outlets in the summer of 1989 and surveyed for aflatoxin B1, zearalenone, and deoxynivalenol (DON [vomitoxin]) by monoclonal antibody-based competitive enzyme-linked immunosorbent assay (ELISA). Only one sample (buckwheat flour) was found to contain aflatoxin B1 (12 ng/g), whereas zearalenone was found in 26% of the samples at a mean concentration of 19 ng/g. In contrast, the DON ELISA was positive in 50% of the samples at a detection level of 1.0-mu-g/g. Between 63 and 88% of corn cereals, wheat flour/muffin mixes, rice cereals, and corn meal/muffin mixes yielded positive results for DON, whereas 25 to 50% of oat cereals, wheat- and oat-based cookies/crackers, corn chips, popcorn, and mixed-grain cereals were positive for DON. The mean DON content of the positive samples was 4.0-mu-g/g, and the minimum and maximum levels were 1.2 and 19-mu-g/g, respectively. When positive ELISA samples were also analyzed by high-performance liquid chromatography, a strong correlation between the two methods was found. The presence of DON in the two highest samples, corn meal and mixed-grain cereal, which contained 19 and 16-mu-g/g, respectively, was quantitatively confirmed by gas chromatography-mass spectrometry. The results indicated that DON was present in 1989 retail food products at concentrations that exceeded those found in previous market surveys and that have been experimentally associated with impaired animal health.