Protein adsorption at the interface between charged polymer substrata and migrating osteoblasts.
Protein adsorption at the interface between charged polymer substrata and migrating osteoblasts.
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DOI：
10.1016/0142-9612(88)90065-8
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发表时间：
1988
期刊：
影响因子：
14
通讯作者：
R. Shelton;A. C. Rasmussen;J. Davies
中科院分区：
文献类型：
--
作者：
R. Shelton;A. C. Rasmussen;J. Davies
用扫描电镜（SEM）和透射电镜（TEM）观察了新生大鼠颅骨成骨细胞在带正电荷和负电荷聚合物基质上的迁移形态。从培养基吸附到这些带电基质上的蛋白质使用2%SDS解吸，并使用聚丙烯酰胺凝胶电泳（PAGE）分离。基质的电荷符号影响蛋白质吸附和成骨细胞迁移形态。细胞变平，适应如此密切的正电荷基质，腹侧细胞膜不能区分在TEM。在带负电荷的基质上，腹侧细胞膜很容易看到，只有局部区域与基质密切接触。腹侧细胞外间隙的深度随负基质上的表面电荷载体种类而变化。两个解吸的蛋白质组分（MW 220和30千道尔顿，分别）与成骨细胞传播的正电荷和负电荷的表面分别。另一种蛋白质组分是唯一存在于从带负电荷的底物解吸的PAGE图谱上。结论：成骨细胞迁移形态受特异性吸附蛋白质的介导.
TheIn vitromigratory morphology of neonate rat calvarial osteoblasts on positively or negatively-charged polymer substrata was observed using scanning (SEM) and transmission electron microscopy (TEM). Proteins adsorbed from the culture medium onto these charged substrata were desorbed using 2% SDS and separated using polyacrylamide gel electrophoresis (PAGE). The charge sign of the substrata affected both protein adsorption and osteoblast migratory morphology. Cells flattened and adapted so closely to positively-charged substrata that the ventral cell membrane could not be distinguished at TEM. On negatively-charged substrata the ventral cell membrane was readily visible, with only focal areas of close contact with the substratum. The depth of the ventral extracellular space varied with the surface charge carrier species on the negative substrata. Two desorbed protein fractions (MW 220 and 30 kDaltons, respectively) were correlated with osteoblast spreading on positive and negatively-charged surfaces respectively. Another protein fraction was uniquely present on PAGE profiles desorbed from negatively charged substrata. It was concluded that the migratory morphology of osteoblasts was influencedviathe intermediary of specifically adsorbed proteins.