Evaluation of the β-barrel outer membrane protein VP1243 as a candidate antigen for a cross-protective vaccine against Vibrio infections
Evaluation of the β-barrel outer membrane protein VP1243 as a candidate antigen for a cross-protective vaccine against Vibrio infections
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β-桶外膜蛋白 VP1243 作为针对弧菌感染的交叉保护疫苗候选抗原的评估
DOI：
10.1016/j.micpath.2020.104419
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发表时间：
2020-10-01
影响因子：
3.8
通讯作者：
Pan, Jianyi
中科院分区：
文献类型：
--
作者：
Gao, Zhichao;Ye, Chen;Pan, Jianyi
副溶血性弧菌是一种革兰氏阴性嗜盐菌，在食用受污染的食物、伤口感染和癫痫发作后会引起急性胃肠炎。抗菌素治疗是控制弧菌感染的主要方法，但弧菌感染不可避免地会产生耐药性。因此，迫切需要一种疫苗来避免这个问题。外膜蛋白(OMP)在宿主免疫系统与细菌的相互作用中起着关键作用。VP1243是副溶血性弧菌的OMP，我们前期研究发现它具有免疫原性。本研究发现VP1243在主要弧菌中分布广泛，高度保守，具有相似的表面表位。该蛋白能刺激副溶血弧菌、溶藻弧菌和创伤弧菌产生较强的抗体反应和交叉免疫反应。值得注意的是，在用VP1243免疫的小鼠中，它对这三种弧菌的致命攻击提供了100%的免疫保护。在免疫的小鼠中观察到这三种弧菌的细胞被有效清除。这些发现为VP1243作为开发预防弧菌感染的多功能疫苗的有前途的候选者提供了确凿的证据。
Vibrio parahaemolyticus is a Gram-negative halophilic bacterium that causes acute gastroenteritis after the consumption of contaminated food, wound infection, and seizures. Antibiotic therapy is the main method for controlling Vibrio infections, which inevitably leads to drug resistance. Therefore, a vaccine is urgently needed to avoid this problem. Outer membrane proteins (OMPs) play a pivotal role in the interaction between the host immune system and bacteria. VP1243 is an OMP of V. parahaemolyticus, and it possessed immunogenicity in our previous study. The present study found that VP1243 was widely distributed, highly conserved and possessed similar surface epitopes among the major Vibrio species. The protein stimulated a strong antibody response and induced cross-reactive immune responses in V. parahaemolyticus, V. alginolyticus and V. vulnificus. Notably, it provided 100% immune protection against lethal challenges by the three Vibrio species in mice immunized with VP1243. Efficient clearance of cells of the three Vibrio bacterial species was observed in immunized mice. These findings provide solid evidence for VP1243 as a promising candidate for the development of a versatile vaccine to protect against Vibrio infections.