Premature onset of fast axonal transport in bromophenylacetylurea neuropathy: an electrophoretic analysis of proteins exported into motor nerve.
Premature onset of fast axonal transport in bromophenylacetylurea neuropathy: an electrophoretic analysis of proteins exported into motor nerve.
复制标题
溴苯乙酰脲神经病中快速轴突运输的过早发作：输出到运动神经的蛋白质的电泳分析。
DOI：
10.1016/0006-8993(90)90315-3
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发表时间：
1990
期刊：
影响因子：
2.9
通讯作者：
Brimijoin,S
中科院分区：
文献类型：
--
作者：
Oka,N;Brimijoin,S
为了检验快速轴突运输蛋白的异常加工是否参与了BPAU（对溴苯乙基脲）诱导的神经病变，我们检查了运输的开始。[35S] BPAU后2周大鼠腰前角注射蛋氨酸，400 mg/kg （i.p）或载药。每隔30-90分钟，消化l4和l5腹根的连续3毫米片段，进行聚丙烯酰胺凝胶电泳和荧光照相。通过与放射标记后结扎16 h的股中坐骨神经样品进行比较，鉴定出快速转运蛋白。一条突出的26 kDa条带代表了最早的输出蛋白。通常在30 min时消失，但在45 min时进入根部。BPAU神经（n= 11）与对照组（n= 11）相比，该束持续移位更远。平均差异为5±0.6mmP< 0.001)。然而，在表观传输速度上没有差异。这些结果提示BPAU神经病的转运早发性。
To test whether abnormal processing of proteins for fast axonal transport is involved in the neuropathy induced by BPAU (p-bromophenylacetylurea) we examined transport onset. [35S]Methionine was injected into the lumbar ventral horn of rats 2 weeks after BPAU, 400 mg/kg (i.p.) or vehicle. At intervals of 30–90 min consecutive 3-mm segments of the L4and L5ventral roots were digested for polyacrylamide gel electrophoresis and fluorography. Fast transported proteins were identified by comparison with samples from mid-thigh sciatic nerve ligated for 16 h after radiolabeling. A prominent 26 kDa band represented the earliest exported protein. It was usually absent at 30 min, but it entered the roots by 45 min. This band was consistently displaced further in BPAU nerve (n= 11) than in controls (n= 11). The mean difference was 5 ± 0.6mmP< 0.001). However, there was no difference in the apparent velocity of transport. These results imply premature onset of transport in BPAU neuropathy.