Capture and Release Erythrocyte from the Blood with Thermoresponsive and Core‐Sheath PCL/PNIPAAm Nanofibers
Capture and Release Erythrocyte from the Blood with Thermoresponsive and Core‐Sheath PCL/PNIPAAm Nanofibers
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DOI：
10.1002/admi.201500652
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发表时间：
2016-03
影响因子：
5.4
通讯作者：
Q. Shi;Jianwen Hou;Xiaodong Xu;Jian Gao;Chunming Li;Jing Jin;S. Wong;Jinghua Yin
中科院分区：
文献类型：
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作者：
Q. Shi;Jianwen Hou;Xiaodong Xu;Jian Gao;Chunming Li;Jing Jin;S. Wong;Jinghua Yin
展示了一种新开发的用于非黏附细胞捕获和释放的纳米纤维平台。将牛血清白蛋白(BSA)偶联的聚N-异丙基丙烯酰胺(PNIPAAm)与聚己内酯共混，通过单喷丝板静电纺丝制备了纳米纤维平台。纳米纤维具有以PNIPAAm为鞘的核-鞘结构，这使得纳米纤维可以随着温度的变化在亲水性和疏水性之间切换。由于牛血清白蛋白固定在鞘部分，纳米纤维抵抗血小板在血液中的粘连，便于直接从血液中捕获和分离红细胞(RBCs)。同时，在温度刺激下，捕获的红细胞很容易从纳米纤维中释放出来。在保持细胞完整性和功能的同时，实现了高达100%的捕获和释放效率。这项工作为后续的分子分析和疾病诊断提供了一个有效捕获和释放非贴壁细胞的平台。
A newly developed nanofiber platform for nonadherent cell capture and release is demonstrated. The nanofiber platform is fabricated by single‐spinneret electrospinning of bovine serum albumin (BSA)‐conjugated poly(N‐isopropylacrylamide) (PNIPAAm) and polycaprolactone blends. The nanofibers possess core‐sheath structure with PNIPAAm as the sheath, which render the nanofibers switchable between hydrophilicity and hydrophobicity with the temperature change. As a result of BSA immobilization on the sheath part, the nanofibers resist platelet adhesion in the blood, facilitating the direct capture and isolation of red blood cells (RBCs) from the blood. Meanwhile, the captured RBCs are readily released from the nanofibers with the temperature stimuli. The capture and release efficiencies of up to 100% are achieved while maintaining cellular integrity and function. This work presents a promising platform to capture and release nonadherent cell effectively for subsequent molecular analysis and disease diagnosis.