Detection of silver nanoparticles in parsley by solid sampling high-resolution-continuum source atomic absorption spectrometry
Detection of silver nanoparticles in parsley by solid sampling high-resolution-continuum source atomic absorption spectrometry
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DOI：
10.1007/s00216-013-7510-0
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发表时间：
2014-06-01
影响因子：
4.3
通讯作者：
Leopold, Kerstin
中科院分区：
文献类型：
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作者：
Feichtmeier, Nadine S.;Leopold, Kerstin
在这项工作中，我们提出了一种通过固体采样高分辨率连续源原子吸收光谱法 (HR-CS AAS) 检测生物材料（欧芹）中银纳米粒子 (AgNP) 的快速而简单的方法。开发了一种新的评估策略，以区分 AgNP 和离子银以及确定 AgNP 的尺寸。为此，引入雾化延迟作为 AgNP 的重要指标，而雾化速率则可以区分 20、60 和 80 nm AgNP。发现含有银离子的样品的雾化延迟比含有银纳米颗粒的样品更高。在优化 AAS 炉程序后，获得了按样品重量归一化的最大雾化延迟差异 6.27 +/- 0.96 s mg(-1)。为此，采用了改变雾化温度、雾化加热速率和热解温度的多变量实验设计。雾化率计算为吸光度信号第一个拐点的斜率，并与生物样品中 AgNP 的大小相关。因此，固体采样 HR-CS AAS 被证明是直接在固体生物样品中识别和区分银纳米粒子与离子银的有前途的工具。
In this work, we present a fast and simple approach for detection of silver nanoparticles (AgNPs) in biological material (parsley) by solid sampling high-resolution-continuum source atomic absorption spectrometry (HR-CS AAS). A novel evaluation strategy was developed in order to distinguish AgNPs from ionic silver and for sizing of AgNPs. For this purpose, atomisation delay was introduced as significant indication of AgNPs, whereas atomisation rates allow distinction of 20-, 60-, and 80-nm AgNPs. Atomisation delays were found to be higher for samples containing silver ions than for samples containing silver nanoparticles. A maximum difference in atomisation delay normalised by the sample weight of 6.27 +/- 0.96 s mg(-1) was obtained after optimisation of the furnace program of the AAS. For this purpose, a multivariate experimental design was used varying atomisation temperature, atomisation heating rate and pyrolysis temperature. Atomisation rates were calculated as the slope of the first inflection point of the absorbance signals and correlated with the size of the AgNPs in the biological sample. Hence, solid sampling HR-CS AAS was proved to be a promising tool for identifying and distinguishing silver nanoparticles from ionic silver directly in solid biological samples.