Symmetric Division and Expression of Its Regulatory Gene Numb in Human Cervical Squamous Carcinoma Cells
Symmetric Division and Expression of Its Regulatory Gene Numb in Human Cervical Squamous Carcinoma Cells
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DOI：
10.1159/000209393
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发表时间：
2009-01-01
期刊：
影响因子：
5
通讯作者：
Xie, Xing
中科院分区：
文献类型：
--
作者：
Chen, Huaizeng;Chen, Xiaojun;Xie, Xing
目的：探讨宫颈鳞状细胞的分裂方式及Numb蛋白的表达。方法：检测了50例宫颈鳞癌（CSCC）、59例宫颈上皮内瘤变（CIN）Ⅱ ~ Ⅲ级、21例CIN Ⅰ级和48例对照组的宫颈鳞状上皮基底细胞卵裂角和Numb蛋白的表达。结果：我们发现CIN I级水平分裂增加，CIN II-III级水平分裂减少。从对照组到CSCC组，Numb表达有增强的趋势。在CSCC中，Numb-PRRL同种型的mRNA表达显著较高，但Numb-PRRS同种型的mRNA表达不高。结论：我们的研究结果表明，在CSCC的初始阶段，癌细胞可能获得增加的对称分裂通过改变分裂方向和增殖。宫颈鳞状上皮中Numb蛋白的表达和Numb-PRRL亚型的表达增加可能是参与CSCC发生的机制之一。版权所有（C）2009 S. Karger AG，巴塞尔
Objective: To investigate the mode of cell division and Numb expression in cervical squamous cells. Method: We measured the cleavage angles of cervical squamous epithelia basal cells and Numb protein expression in 50 cases of cervical squamous cell carcinomas (CSCC), 59 cervical intraepithelial neoplasias (CIN) grade II-III, 21 CIN grade I and 48 controls. Results: We found an increase in horizontal divisions in CIN grade I and a decrease in horizontal divisions in CIN grade II-III. Numb expression had a tendency to be stronger from control to CSCC. In CSCC there was a significantly higher expression of mRNA for the Numb-PRRL isoform but not for the Numb-PRRS isoform. Conclusion: Our findings suggest that in the initial stage of CSCC, carcinomatous cells may acquire an increase of symmetric division by altering cleavage orientation and proliferation. Expression of Numb protein in Cervical squamous epithelia and an increased expression of Numb-PRRL isoforms may be among the mechanisms involved in the genesis of CSCC. Copyright (C) 2009 S. Karger AG, Basel