Parallelism and Epistasis in Skeletal Evolution Identified through Use of Phylogenomic Mapping Strategies
Parallelism and Epistasis in Skeletal Evolution Identified through Use of Phylogenomic Mapping Strategies
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DOI：
10.1093/molbev/msv208
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发表时间：
2016-01-01
影响因子：
10.7
通讯作者：
Harris, Matthew P.
中科院分区：
文献类型：
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作者：
Daane, Jacob M.;Rohner, Nicolas;Harris, Matthew P.
识别进化变化背后的遗传机制对于我们理解自然多样性至关重要，但目前大多数物种缺乏遗传和基因组资源。在这里，我们提出了一个新的比较基因组学的方法，可以应用到广泛的分类学采样的非模式物种的进化变化的遗传基础进行调查。使用我们的分析管道，我们表明，重复和分歧的fgfr1a与属Phoxinellus鱼类内的鳞片减少。作为一个平行的遗传机制，观察到在规模缩小内的独立谱系的鲤，我们的发现揭示了显着的发展约束指导形态进化。此外，我们确定了Phoxinellus内fgf20 a的固定变异，并证明了斑马鱼内fgfr1a和fgf20 a的组合功能丧失表型模仿了进化的缩放模式。总之，这些发现揭示了fgfr1a和fgf20 a之间的上位相互作用作为一种发育机制，调节鱼类骨骼的变化。
The identification of genetic mechanisms underlying evolutionary change is critical to our understanding of natural diversity, but is presently limited by the lack of genetic and genomic resources for most species. Here, we present a new comparative genomic approach that can be applied to a broad taxonomic sampling of nonmodel species to investigate the genetic basis of evolutionary change. Using our analysis pipeline, we show that duplication and divergence of fgfr1a is correlated with the reduction of scales within fishes of the genus Phoxinellus. As a parallel genetic mechanism is observed in scale-reduction within independent lineages of cypriniforms, our finding exposes significant developmental constraint guiding morphological evolution. In addition, we identified fixed variation in fgf20a within Phoxinellus and demonstrated that combinatorial loss-of-function of fgfr1a and fgf20a within zebrafish phenocopies the evolved scalation pattern. Together, these findings reveal epistatic interactions between fgfr1a and fgf20a as a developmental mechanism regulating skeletal variation among fishes.