Leukotriene C promotes prostacyclin synthesis by human endothelial cells.
Leukotriene C promotes prostacyclin synthesis by human endothelial cells.
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白三烯 C 促进人内皮细胞合成前列环素。
DOI：
10.1073/pnas.80.13.4109
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发表时间：
1983
影响因子：
11.1
通讯作者：
Scott,WA
中科院分区：
文献类型：
--
作者：
Cramer,EB;Pologe,L;Pawlowski,NA;Cohn,ZA;Scott,WA
用[~ 3 H]花生四烯酸标记培养的人脐静脉内皮细胞16小时，标记物主要分布在磷脂中（93%），73%均匀分布在磷脂酰胆碱和磷脂酰乙醇胺之间。白三烯C（10- 1，000 nM）促进放射性标记以剂量依赖性方式释放到培养基中。在100 nM时，该响应是对照值的3.3倍。合成的主要花生四烯酸代谢产物是前列环素，占总释放放射性标记的33%。内皮细胞也释放少量的前列腺素F2 α（6.1%），不明脂氧合酶产物（14.8%），和未反应的花生四烯酸（33%）。释放的30分钟的时间过程是独立的白三烯C浓度使用。相似浓度的白三烯D也促进内皮细胞主要释放前列环素和未反应的花生四烯酸。前列环素是一种有效的血管扩张剂，其释放可能是慢反应物质对血管系统作用的重要介质。
Cultured endothelial cells from human umbilical vein were labelled with [3H]arachidonic acid for 16 hr. The radiolabel was localized primarily in phospholipids (93%) and 73% was distributed equally between phosphatidylcholine and phosphatidylethanolamine. Leukotriene C (10-1,000 nM) promoted a dose-dependent release of radiolabel into the culture medium. This response was 3.3 times control values at 100 nM. The major arachidonic acid metabolite synthesized was prostacyclin, which was 33% of the total released radiolabel. Endothelial cells also released small amounts of prostaglandin F2 alpha (6.1%), unidentified lipoxygenase products (14.8%), and unreacted arachidonic acid (33%). The 30-min time course of release was independent of the leukotriene C concentration used. Leukotriene D at similar concentrations also promoted endothelial cells to release primarily prostacyclin and unreacted arachidonic acid. The release of prostacyclin, a potent vasodilator agent, may be an important mediator in slow reacting substance effects on the vasculature.